{"product_id":"abcam-ab100785","title":"Abcam, ab100785, Rat TNF alpha ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nRat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat TNF alpha in Rat in Cell\/Tissue Extracts, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts, Cell culture supernatant,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 25 pg\/mL,\u003cbr\u003e\nRange:82.3 - 20000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's TNF alpha ELSA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in cell lysates and tissue lysates.\u003cbr\u003e\nThis assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843544141993,"sku":"ab100785","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab100785","provider":"Iright","version":"1.0","type":"link"}