{"product_id":"abcam-ab10152","title":"Abcam, ab10152, Anti-p114RhoGEF antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nGoat Polyclonal p114RhoGEF antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGEF18 aa 1000-1050.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Goat,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human ARHGEF18 aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information.Q6ZSZ5\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nRho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nP114RhoGEF also known as ARHGEF18 is a guanine nucleotide exchange factor (GEF) specific for Rho GTPases. It functions by facilitating the exchange of GDP for GTP on RhoA a process important for activating RhoA. p114RhoGEF is approximately 114 kDa in mass. It is expressed in various tissues including vascular endothelial cells and neurons indicating a diverse range of functional roles across different systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein plays an important role in the regulation of the actin cytoskeleton dynamics and cell migration. p114RhoGEF associates with other proteins to form complexes that regulate cell shape and motility. It binds with G-proteins and other signaling molecules influencing cellular responses to environmental cues such as changes in cell adhesion or polarity.\u003cbr\u003e\nPathways\u003cbr\u003e\nP114RhoGEF is involved in the Rho signaling pathway which is essential for maintaining cell structure and function. Within this pathway it interacts closely with proteins like ZO-1 and Gα12\/13 to control stress fiber formation and focal adhesion. It also features in pathways regulating epithelial cell junctions and endothelial barrier integrity which are critical for tissue homeostasis and vascular function.\u003cbr\u003e\nP114RhoGEF plays a notable role in cancer progression and cardiovascular diseases. Disruptions in its function can lead to aberrant cell migration and invasion factors linked to tumor metastasis. Additionally changes in its activity might influence endothelial barrier function contributing to vascular leakiness associated with inflammatory conditions. Related proteins such as RhoA and ZO-1 also partake in these pathological processes highlighting the significance of p114RhoGEF in health and disease contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854428623017,"sku":"ab10152","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab10152","provider":"Iright","version":"1.0","type":"link"}