{"product_id":"abcam-ab102524","title":"Abcam, ab102524, Lipase Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nLipase Assay Kit ab102524 is a rapid, simple, and sensitive colorimetric assay to quantify lipase activity. Readout on any colorimetric (570 nm) plate reader. - Kit includes Lipase positive control\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue Extracts, Cell culture media, Cell culture extracts, Milk, Serum,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:= 0.02 mU\/well,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nLipase assay kit measure the activity of lipase in a sample by using a reaction that produces a color or fluorescence. The amount of the color or fluorescence produced is proportional to the amount of lipase in the sample. In the lipase assay protocol, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by monitoring a linked change in the absorbance of a probe (OD=570nm). This kit is suitable for high throughput analysis (HTP).\u003cbr\u003e\nThis lipase assay kit detects lipase activity as low as 0.02mU per well.\u003cbr\u003e\nLipase Assay Kit (Colorimetric)summary:\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Prepare standard curve\u003cbr\u003e\n- Add reaction mix\u003cbr\u003e\n- Measure optical density (OD570 nm) in a kinetic mode for 60-90 min at 37°C on a microplate reader\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nLipase Assay Kit (Colorimetric) has been used in a variety of sample type including:\u003cbr\u003e\nMouse colon and pancreas samples\u003cbr\u003e\nMouse lung cancer samples\u003cbr\u003e\nBlood samples from mice with pancreatitis\u003cbr\u003e\nReferences:\u003cbr\u003e\n1-Xia H et al. 2023\u003cbr\u003e\n2-Bartolacci C et al. 2022\u003cbr\u003e\n3-Tarasiuk A et al. 2021\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee other alternative kits to quantify lipase activity :\u003cbr\u003e\n- Lipase Assay Kit III (Fluorometric)\u003cbr\u003e\nab118969\u003cbr\u003e\n- Lipase Assay Kit II (Colorimetric)\u003cbr\u003e\nab102525\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K722 Lipase Activity Colorimetric Assay Kit. K722-100 is the same size as the 100 test size of ab102524.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843611611305,"sku":"ab102524","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab102524","provider":"Iright","version":"1.0","type":"link"}