{"product_id":"abcam-ab102530","title":"Abcam, ab102530, Glutathione Peroxidase Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test \/ 2000Test\u003cbr\u003e\nGlutathione Peroxidase Assay Kit (Colorimetric) ab102530 can be used to quantitate the activity of all of the glutathione dependent peroxidases present in samples of plasma, tissue extracts, cell culture supernatant, serum, platelets, and other biological fluids. Readout on any colorimetric (340 nm) microplate reader. - Results in just 40 min - Ease of use - rated excellent by customers - Trusted by the scientific community - cited in \u0026gt;140 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Platelets, Other biological fluids,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:= 0.5 mU\/mL,\u003cbr\u003e\nAssay time:40m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nGlutathione peroxidase assay kit measures the activity of glutathione peroxidase by measuring the decrease in absorbance of NADPH. The assay is based on the oxidation of reduced glutathione to oxidized glutathione.\u003cbr\u003e\nIn the glutathione peroxidase assay protocol, glutathione peroxidase (GPx) oxidizes GSH to produce GSSG as part of the reaction in which it reduces cumene hydroperoxide. Glutathione reductase (GR) then reduces the GSSG to produce GSH, and in the same reaction consumes NADPH. The decrease of NADPH (measured at OD=340 nm) is proportional to GPx activity.\u003cbr\u003e\nGlutathione peroxidase assay protocol summary:\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add reaction mix and incubate for 15 min at room temp to deplete all GSSG in sample\u003cbr\u003e\n- Add cumene hydroperoxide\u003cbr\u003e\n- Analyze with microplate reader immediately, and after at least 5 min\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nGlutathione Peroxidase Assay Kit (Colorimetric) has been used in a variety of sample type including:\u003cbr\u003e\nMouse C2C12 myoblasts\u003cbr\u003e\nMice heart samples\u003cbr\u003e\nRats gonads homogenates\u003cbr\u003e\nReferences:\u003cbr\u003e\n1-Martin S et al. 2023\u003cbr\u003e\n2-Li H et al. 2023\u003cbr\u003e\n3-Naguib G et al. 2023\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nFor fluorometric detection, we recommend Glutathione Peroxidase Activity Assay Kit (Fluorometric)\u003cbr\u003e\nab219926\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K762 Glutathione Peroxidase Activity Colorimetric Assay Kit. K762-100 is the same size as the 100 test size of ab102530.\u003cbr\u003e\nThe Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes plays and important role in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlutathione Peroxidase (GPx) often referred to as GSH peroxidase is an essential enzyme in the cellular defense system against oxidative damage. It exists as a group of enzymes including the widely studied isoform GPx4. GPx has a molecular weight of approximately 20-23 kDa varying slightly depending on the isoform. This enzyme is localized in many tissues across different organs including the liver kidney and muscles. It catalyzes the reduction of hydrogen peroxide and organic hydroperoxides using glutathione (GSH) as a substrate preventing the formation of free radicals.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme plays a significant role in the detoxification of reactive oxygen species. GPx does not usually form a complex with other proteins; instead it acts individually within cells to maintain the balance of oxidative and reductive forces. By converting harmful peroxides into non-toxic alcohols and water it protects cell membranes and other cellular components from oxidative damage. GPx activity is often measured in laboratories using assays to determine its efficiency in catalyzing these reactions.\u003cbr\u003e\nPathways\u003cbr\u003e\nGPx engages in critical cellular antioxidant defense mechanisms. It is an integral part of the glutathione redox cycle where it partners with glutathione reductase another enzyme important for regenerating reduced glutathione. Additionally GPx links to the pentose phosphate pathway providing reduced NADPH necessary for maintaining glutathione in its reduced form. These pathways protect cells from oxidative stress and keep redox homeostasis in check.\u003cbr\u003e\nAlterations in GPx activity correlate with various health conditions. Researchers link decreased GPx activity to cardiovascular diseases and neurodegenerative disorders highlighting its importance in maintaining cellular health. In cardiovascular diseases the imbalance of oxidative stress pathways involves proteins like superoxide dismutase in addition to GPx worsening the effects of oxidative damage. In neurodegenerative disorders abnormal protein aggregations disturb GPx activities emphasizing the role of antioxidants in disease prevention and management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843539423401,"sku":"ab102530","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab102530","provider":"Iright","version":"1.0","type":"link"}