{"product_id":"abcam-ab102859","title":"Abcam, ab102859, APC\/Cy7® Conjugation Kit - Lightning-Link®","description":"\u003cp\u003eSize: 100µg \/ 3 x 10µg \/ 3 x 100µg \/ 1mg\u003cbr\u003e\nAPC\/Cy7® Conjugation Kit - Lightning-Link® (ab102859) offers several standout features: - Rapid Conjugation: achieve APC\/Cy7 labeling in under 4 hours with just 30 seconds of hands-on time. - High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies. - Versatility: suitable for conjugating antibodies, proteins, and peptides. APC\/Cy7 labeled antibodies can be used immediately in applications such as Flow cytometry , ELISA, and Immunohistochemistry (IHC) without further purification. - Confidence: cited in over 20 publications.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nAPC\/Cy7®Conjugation Kit - Lightning-Link (ab102859) uses a simple and quick process for APC\/Cy7 labeling \/ conjugation of antibodies. It can also be used to conjugate other proteins or peptides.\u003cbr\u003e\nTo conjugate an antibody to APC\/Cy7® using this kit:\u003cbr\u003e\n- add modifier to antibody and incubate for 3 hrs\u003cbr\u003e\n- add quencher and incubate for 30 mins\u003cbr\u003e\nThe APC\/Cy7® conjugated antibody can be used immediately in Flow cytometry and other applications. No further purification is required and 100% of the antibody is recovered for use.\u003cbr\u003e\nThe excitation and emission wavelengths for APC\/Cy7® are Ex: 650 nm, Em: 774 nm.\u003cbr\u003e\nLearn about our antibody labeling kits and their advantages\u003cbr\u003e\nLearn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our\u003cbr\u003e\nantibody purification and concentration kits\u003cbr\u003e\nCustom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.\u003cbr\u003e\nAmount and volume of antibody for conjugation to APC\/Cy7\u003cbr\u003e\nKit size\u003cbr\u003e\nRecommended maximum\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum antibody\u003cbr\u003e\nvolume\u003cbr\u003e\n3 x 10 µg\u003cbr\u003e\n3 x 10 µg\u003cbr\u003e\n3 x 10 µL\u003cbr\u003e\n100 µg\u003cbr\u003e\n1 x 100 µg\u003cbr\u003e\n1 x 100 µL\u003cbr\u003e\n3 x 100 µg\u003cbr\u003e\n3 x 100 µg\u003cbr\u003e\n3 x 100 µL\u003cbr\u003e\n1 x 1 mg\u003cbr\u003e\n1 x 1 mL\u003cbr\u003e\nIdeal antibody concentration is 1 mg\/mL. 0.5 - 1 mg\/mL can be used if the maximum antibody volume is not exceeded. Antibodies \u0026gt; 1 mg\/mL or \u0026lt; 0.5 mg\/mL should be diluted \/concentrated.\u003cbr\u003e\nBuffer Requirements for Conjugation\u003cbr\u003e\nBuffer should be pH 6.5-8.5.\u003cbr\u003e\nCompatible buffer constituents\u003cbr\u003e\nIf a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.\u003cbr\u003e\n50 mM \/ 0.6% Tris\u003cbr\u003e\n0.1% BSA\u003cbr\u003e\n50% glycerol\u003cbr\u003e\n0.1% sodium azide\u003cbr\u003e\nPotassium phosphate\u003cbr\u003e\nSodium chloride\u003cbr\u003e\nSucrose\u003cbr\u003e\nSodium citrate\u003cbr\u003e\nTrehalose\u003cbr\u003e\nTris buffered saline is almost always ≤ 50 mM \/ 0.6%\u003cbr\u003e\nIncompatible buffer constituents\u003cbr\u003e\nThiomerosal\u003cbr\u003e\nProclin\u003cbr\u003e\nGlycine\u003cbr\u003e\nArginine\u003cbr\u003e\nGlutathione\u003cbr\u003e\nIf a constituent of the buffer containing your antibody or protein is not listed above, please contact us.\u003cbr\u003e\nOnly purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.\u003cbr\u003e\nStoring and handling conjugation kits\u003cbr\u003e\nLyophilized Lightning-Link\u003cbr\u003e\ncomponents are hygroscopic.\u003cbr\u003e\nKits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link\u003cbr\u003e\nAPC\/Cy7 Labeling Kit. 765-0015 is the same as the 1 mg size. 765-0010 is the same as the 3 x 100 µg size. 765-0030 is the same as the 3 x 10 µg size. 765-0005 is the same as the 100 µg size.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847593087145,"sku":"ab102859","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab102859","provider":"Iright","version":"1.0","type":"link"}