{"product_id":"abcam-ab103741","title":"Abcam, ab103741, StayRed\/AP 30ml (Alcohol \u0026 Xylene Compatible)","description":"\u003cp\u003eSize: 30mL\u003cbr\u003e\nStayRed\/AP (ab103741) is a substrate chromogen system designed for either IHC or ISH when using alkaline phosphate (AP) detection. - Compatible with Alcohol \u0026amp; Xylene solutions\u003cbr\u003e\nProduct details:\u003cbr\u003e\nStayRed\/AP (ab103741) is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayRed\/AP produces a brilliant dark red color. It is also insoluble in organic solvents so sections can be dehydrated in alcohol, cleared in xylene (or a xylene substitute) and permenently mounted.\u003cbr\u003e\nIntended Use:\u003cbr\u003e\nSubstrate\/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.\u003cbr\u003e\nWorking Solution:\u003cbr\u003e\nAliquot 3mL of StayRed\/AP Substrate Buffer in a mixing bottle. Add one drop (~20 μL) of StayRed\/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.\u003cbr\u003e\nNote: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.\u003cbr\u003e\nProcedure:\u003cbr\u003e\nAfter SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.\u003cbr\u003e\nWipe slides, removing excess buffer. Add enough drops of StayRed\/AP working solution to cover tissue sections.\u003cbr\u003e\nIncubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.\u003cbr\u003e\nCounterstain sections in hematoxylin.\u003cbr\u003e\nDehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.\u003cbr\u003e\nFind complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the\u003cbr\u003e\nIHC kits and reagents guide\u003cbr\u003e\nIntended Use:\u003cbr\u003e\nSubstrate\/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.\u003cbr\u003e\nWorking Solution:\u003cbr\u003e\nAliquot 3mL of StayRed\/AP Substrate Buffer in a mixing bottle. Add one drop (~20 μL) of StayRed\/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.\u003cbr\u003e\nNote: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.\u003cbr\u003e\nProcedure:\u003cbr\u003e\nAfter SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.\u003cbr\u003e\nWipe slides, removing excess buffer. Add enough drops of StayRed\/AP working solution to cover tissue sections.\u003cbr\u003e\nIncubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.\u003cbr\u003e\nCounterstain sections in hematoxylin.\u003cbr\u003e\nDehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847601279145,"sku":"ab103741","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab103741","provider":"Iright","version":"1.0","type":"link"}