{"product_id":"abcam-ab104139","title":"Abcam, ab104139, Mounting Medium With DAPI - Aqueous, Fluoroshield","description":"\u003cp\u003eSize: 20mL \/ 100mL\u003cbr\u003e\nDAPI mounting medium (ab104139) is a ready-to-use aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. Suitable to prevent rapid photobleaching for IHC, ISH and ICC\/IF applications. - Proven performance: cited in over 325 publications - Compatible with our range of fluorescent labeled secondary antibodies - Available in different sizes to fit your experimental needs\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:ICC\/IF, IHC-P, ISH, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Preservative: 0.09% Sodium azideConstituents: 84% Water, 0.0002% 4',6-Diamidino-2-phenylindole dihydrochloride\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nFluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC.\u003cbr\u003e\nThis mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.\u003cbr\u003e\nMounting media products\u003cbr\u003e\nFor fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium\u003cbr\u003e\nab104135\u003cbr\u003e\n, Mounting Medium with PI\u003cbr\u003e\nab104129\u003cbr\u003e\n, and this product, Mounting Medium with DAPI ab104139. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI\u003cbr\u003e\nab188804\u003cbr\u003e\nFor chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend aqueous Mounting Medium\u003cbr\u003e\nab64230\u003cbr\u003e\n, or organic Limonene Mounting Medium\u003cbr\u003e\nab104141\u003cbr\u003e\nProcedure\u003cbr\u003e\nBring the vial to room temperature.\u003cbr\u003e\nRinse slide to be mounted with distilled or deionized, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.\u003cbr\u003e\nTurn the vial upside down and open the dropper to remove any air bubbles.\u003cbr\u003e\nApply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.\u003cbr\u003e\nLet stand at room temperature for about 5 minutes.\u003cbr\u003e\nApply cover slip carefully avoiding air bubbles.\u003cbr\u003e\nThe specimen is ready for visualization under a microscope.\u003cbr\u003e\nOne can seal the edges of cover slip with nail polish, any organic medium or our Organo mounting medium (\u003cbr\u003e\nab104141\u003cbr\u003e\n). If a coverslip is not sealed air bubbles will appear in few days.\u003cbr\u003e\nFor storage it is recommended that the slide be stored in the dark at 2-8°C.\u003cbr\u003e\nRemoval of Coverslip\u003cbr\u003e\nCoverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.\u003cbr\u003e\nRefractive Index\u003cbr\u003e\n1.364 ± 0.002. This number applies to this mounting medium in solution. Refractive indexes change when the water solvent evaporates and mounting media dries on slides. We do not have the means to measure the refractive indexes of dry mounting mediums; however we expect the numbers to go higher when dried. The refractive index of water is 1.3330.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze, Store in the dark\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847570509993,"sku":"ab104139","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab104139","provider":"Iright","version":"1.0","type":"link"}