{"product_id":"abcam-ab108400","title":"Abcam, ab108400, Anti-Calpain 1 antibody [EPR3319]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-Calpain 1 antibody [EPR3319] (ab108400) is a rabbit monoclonal antibody detecting Calpain 1 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR3319,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IHC-P, WB, ICC\/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody recognises the large subunit of Calpain\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Calpain 1 antibody [EPR3319] (ab108400) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of Calpain 1?\u003cbr\u003e\nAnti-Calpain 1 [EPR3319] (ab108400) specifically detects a band for Calpain 1 (UniProt: P07384) at a molecular weight of 82kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Calpain 1 [EPR3319] (ab108400) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-Calpain 1 antibody [EPR3319] (ab108400) has been confirmed by Western blot testing in Calpain 1 Knockout HAP1 cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR3319] also available for your convenience: ab108400, Carrier free -\u003cbr\u003e\nab239930\u003cbr\u003e\n, PE -\u003cbr\u003e\nab306003\u003cbr\u003e\n, APC -\u003cbr\u003e\nab306004\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab306005\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308851\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab309780\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab310151\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310579\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab312108\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312591\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab320900\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCalpain 1 also known as CAPN1 is a calcium-dependent cysteine protease with a mass of approximately 80 kDa. It exhibits calpain activity by cleaving substrate proteins in a regulated manner. Calpain 1 expresses in various tissues including muscle brain and lens suggesting its involvement in diverse cellular processes. The enzyme is part of a larger calpain family with calpain-1 calpain-2 and other isoforms that demonstrate differing substrate specificities and regulatory mechanisms.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCalpain 1 participates in cellular functions such as signal transduction cell cycle progression and apoptosis. It is not part of a complex but operates independently to modulate cellular responses by processing different target proteins. Calpain protein activity impacts cytoskeletal remodeling and membrane repair important processes in cell mobility and stability. Through its proteolytic actions calpain 1 influences neural plasticity and muscle function demonstrating its broad biological significance.\u003cbr\u003e\nPathways\u003cbr\u003e\nCalpain 1 functions within the calcium signaling and apoptotic pathways highlighting its role in regulating cellular survival and death. Calcium influx triggers calpain activity linking it to proteins such as calmodulin and calcineurin that also respond to calcium signals. Calpain-1 modulates apoptosis by targeting Bcl-2 family proteins influencing cell fate during stress responses. The calpain assay measures its activity providing insights into its contribution to these vital pathways.\u003cbr\u003e\nCalpain 1 connects to neurodegenerative diseases like Alzheimer's and muscular diseases such as muscular dystrophy. Alzheimer's disease features abnormal calpain activity affecting tau protein and amyloid precursor protein processing. In muscular dystrophy calpain-1's unregulated activity leads to excessive muscle degradation aggravating the disease condition. Its interaction with spectrin and dystrophin proteins suggests a mechanistic role in the pathogenesis of these disorders pinpointing calpain-1 as a potential therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844099756201,"sku":"ab108400","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab108400","provider":"Iright","version":"1.0","type":"link"}