{"product_id":"abcam-ab108788","title":"Abcam, ab108788, Human Albumin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Albumin ELISA kit is a sandwich ELISA used to quantify human albumin (ALB) with a sensitivity of 0.89 ng\/mL - Broad sample compatibility – works with urine, saliva, milk, cerebrospinal fluid (CSF), cell culture supernatants, cell lysates, and tissue samples - Wide dynamic range – Detects albumin levels from 3.125–200 ng\/mL - Efficient assay process – Total assay time of approximately 3 hours - Comprehensive kit components: Includes pre-coated 96-well microplate\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue, Cerebral Spinal Fluid, Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.89 ng\/mL,\u003cbr\u003e\nRange:3.125 - 200 ng\/mL,\u003cbr\u003e\nAssay time:3h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Albumin ELISA Kit ab108788 is a sandwich ELISA to measure Human Albumin (ALB) in Tissue, Cerebral Spinal Fluid (CSF), Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate with a sensitivity of 0.79 ng\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nAn albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.\u003cbr\u003e\nHuman Albumin ELISA Kit ab108788 protocol summary\u003cbr\u003e\n1. Add samples or standards to the wells\u003cbr\u003e\n2. Incubate for 1 hour then wash\u003cbr\u003e\n3. Add biotinylated detector antibody\u003cbr\u003e\n4. Incubate for 30 min then wash\u003cbr\u003e\n5. Add the SP-conjugate\u003cbr\u003e\n6. Incubate for 30 min then wash\u003cbr\u003e\n7. Add the chromogen substrate\u003cbr\u003e\n8. Incubate for 25 min\u003cbr\u003e\n9. Add stop solution and read at 450 nm\u003cbr\u003e\nHow other researchers are using Human Albumin ELISA kit ab108788\u003cbr\u003e\nThe Human Albumin ELISA kit ab108788 has been used in publications to assess therapeutic effects of adipose-derived stem cells on acute liver failure\u003cbr\u003e\n, effects of GP130 signaling in liver stenosis\u003cbr\u003e\n, and potential therapeutic targets for diabetic nephropathy\u003cbr\u003e\nReferences:\u003cbr\u003e\nXiaoyan Zhang et al. 2023, PMID: 37078751,\u003cbr\u003e\nZhe Li et al. 2023 PMID: 37058568,\u003cbr\u003e\nLiyu He et al. 2022, PMID: 35813614\u003cbr\u003e\nGet results in 90 minutes our SimpleStep ELISA\u003cbr\u003e\n- Human Albumin ELISA Kit\u003cbr\u003e\nab179887\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman Albumin ELISA kit ab108788 is commonly used by researchers to measure liver or kidney function.\u003cbr\u003e\nLiver function ELISA assays include Human ALT ELISA kit\u003cbr\u003e\nab234578\u003cbr\u003e\nand Human AST ELISA Kit\u003cbr\u003e\nab263881\u003cbr\u003e\nThese ELISA kits are also available for Mouse\/Rat:\u003cbr\u003e\n- Mouse Albumin ELISA kit\u003cbr\u003e\nab207620\u003cbr\u003e\n- Mouse ALT ELISA kit\u003cbr\u003e\nab282882\u003cbr\u003e\n- Mouse AST ELISA kit\u003cbr\u003e\nab263882\u003cbr\u003e\n- Rat Albumin ELISA kit\u003cbr\u003e\nab235642\u003cbr\u003e\n- Rat ALT ELISA kit abab234579\u003cbr\u003e\n- Rat AST ELISA kit\u003cbr\u003e\nab263883\u003cbr\u003e\nKidney function marker assays include:\u003cbr\u003e\n- Creatinine Assay Kit\u003cbr\u003e\nab65340\u003cbr\u003e\n– suitable for use as a serum creatinine assay and a urine creatinine assay.\u003cbr\u003e\n- Albumin (BCG) Assay Kit (Colorimetric)\u003cbr\u003e\nab235628\u003cbr\u003e\n– suitable for use with serum\u003cbr\u003e\n- Urea Assay Kit\u003cbr\u003e\nab83362\u003cbr\u003e\n– suitable for use with serum\/plasma and urine, and used as a blood urea nitrogen assay.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlbumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlbumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.\u003cbr\u003e\nPathways\u003cbr\u003e\nMore than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.\u003cbr\u003e\nResearchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843539554473,"sku":"ab108788","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab108788","provider":"Iright","version":"1.0","type":"link"}