{"product_id":"abcam-ab108793","title":"Abcam, ab108793, Albumin Rabbit ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nAlbumin Rabbit ELISA Kit is a competitive ELISA used to quantify Rabbit Albumin with a sensitivity of 0.15 µg\/mL. - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including serum, plasma, and urine - Wide dynamic range – quantifies 0.195 - 200 µg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Serum,\u003cbr\u003e\nReacts with:Rabbit,\u003cbr\u003e\nAssay type:Competitive,\u003cbr\u003e\nSensitivity:\u0026gt;= 0.15 µg\/mL,\u003cbr\u003e\nRange:0.195 - 200 µg\/mL,\u003cbr\u003e\nAssay time:3h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAlbumin Rabbit ELISA Kit ab108793 is a competitive ELISA kit to measure Rabbit Albumin in Serum, Plasma, and Urine with a sensitivity of 0.15 µg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nRabbit Albumin ELISA Kit ab108793 employs an Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Strepatvidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Strepatvidin-Peroxidase enzymatic reaction. TMB is catalyzed by Strepatvidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native rabbit albumin in serum, plasma, and urine samples.\u003cbr\u003e\nAlbumin Rabbit ELISA Kit ab108793 protocol summary:\u003cbr\u003e\n1. Add standard or sample to each well used and add prepared biotin protein to each well. Incubate at room temperature.\u003cbr\u003e\n2. Wash each well and add Streptavidin Conjugate Solution. Incubate at room temperature.\u003cbr\u003e\n3. Add TMB Solution to each well. Incubate at room temperature.\u003cbr\u003e\n4. Add Stop Solution to each well. Read immediately.\u003cbr\u003e\nHow other researchers are using Albumin Rabbit ELISA Kit ab108793\u003cbr\u003e\nAlbumin Rabbit ELISA Kit ab108793 has been used to study the contribution of renal nerves in rabbit models for chronic kidney disease.\u003cbr\u003e\nReferences:\u003cbr\u003e\nY. Sata, et.al., 2020, PMID: 32895021\u003cbr\u003e\nGet results in 90 minutes with our SimpleStep ELISA\u003cbr\u003e\n- Human Albumin ELISA Kit\u003cbr\u003e\nab227933\u003cbr\u003e\n- Human Albumin ELISA Kit\u003cbr\u003e\nab179887\u003cbr\u003e\n- Mouse Albumin ELISA Kit\u003cbr\u003e\nab207620\u003cbr\u003e\n- Rat Albumin ELISA Kit\u003cbr\u003e\nab235642\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlbumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlbumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.\u003cbr\u003e\nPathways\u003cbr\u003e\nMore than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.\u003cbr\u003e\nResearchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855621574825,"sku":"ab108793","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab108793","provider":"Iright","version":"1.0","type":"link"}