{"product_id":"abcam-ab108823","title":"Abcam, ab108823, Human Complement C3 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Complement C3 ELISA Kit is a Sandwich ELISA used to quantify Human Complement C3 with a sensitivity of 84 pg\/ml - Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Wide dynamic range – 0.625 - 40 ng\/ml - Cited in over 15 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue, Cerebral Spinal Fluid, Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026gt;= 84 pg\/mL,\u003cbr\u003e\nRange:0.625 - 40 ng\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Complement C3 ELISA kit is designed for the quantitative measurement of Complement C3 concentrations in Human urine, milk, saliva, cerebrospinal fluid and cell culture supernatants.\u003cbr\u003e\nA Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003cbr\u003e\nHuman Complement C3 ELISA Kit ab108823 is a sandwich ELISA to measure Human complement C3 in urine, milk, saliva, cerebrospinal fluid and cell culture supernatants with a sensitivity of 84 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nThis kit recognizes Complement C3 in urine, milk, saliva, cerebrospinal fluid, tissue and cell culture supernatant\u003cbr\u003e\nHuman Complement C3 ELISA Kit ab108823 protocol summary\u003cbr\u003e\n1. Add samples and standards to wells. Incubate at room temperature.\u003cbr\u003e\n2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature.\u003cbr\u003e\n3. Wash each well and add Streptavidin Solution. Incubate at room temperature.\u003cbr\u003e\n4. Add TMB Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read immediately.\u003cbr\u003e\nHow other researchers are using Human C3 ELISA kit ab108823\u003cbr\u003e\nHuman C3 ELISA kit ab108823 has been used in the study of the relationsip between microglia and complement in Huntingdon's disease\u003cbr\u003e\nand to assess the function of kidney organoids\u003cbr\u003e\nReferences:\u003cbr\u003e\n: DK Wilton et al. 2023, PMID: 37814059,\u003cbr\u003e\nM Lasse et al., 2023,PMID: 37580326\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman Complement C3 Elisa kit ab108823 is commonly used to assess the ability of the immune system to fight infection.\u003cbr\u003e\nOther ELISA kits to measure complement factors include:\u003cbr\u003e\n- Human Complement factor H ELISA Kit\u003cbr\u003e\nab252359\u003cbr\u003e\n- Human Complement Factor I ELISA Kit\u003cbr\u003e\nab284615\u003cbr\u003e\n- Human C4b ELISA Kit (Complement C4-B)\u003cbr\u003e\nab277717\u003cbr\u003e\n- Human C8a (Complement C8a) ELISA Kit\u003cbr\u003e\nab288173\u003cbr\u003e\n- Human Complement Factor D ELISA Kit\u003cbr\u003e\nab243686\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nComplement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nComplement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.\u003cbr\u003e\nComplement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843542929577,"sku":"ab108823","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab108823","provider":"Iright","version":"1.0","type":"link"}