{"product_id":"abcam-ab108878","title":"Abcam, ab108878, Human Lipoprotein A ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Lipoprotein A ELISA Kit is a sandwich ELISA used to quantify Human Lipoprotein A with a sensitivity of 0.56 ng\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Wide dynamic range – quantifies 1.563 - 50 ng\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cerebral Spinal Fluid, Milk, Plasma, Serum, Urine,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.56 ng\/mL,\u003cbr\u003e\nRange:1.563 - 50 ng\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Lipoprotein A ELISA Kit ab108878 is a sandwich ELISA kit to measure Human Lipoprotein A in Serum, Plasma, Milk, Urine, and Cerebral Spinal Fluid with a sensitivity of 0.56 ng\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman Lipoprotein A ELISA Kit ab108878 employs a Lipoprotein A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lipoprotein A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lipoprotein A captured in plate.br\u0026gt;\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes Lipoprotein A in plasma, serum, urine, milk, and CSF samples.\u003cbr\u003e\nNo significant cross-reactivity observed with Human Apo A1, Apo A2, Apo A4, Apo A5, Apo C1, Apo C2, Apo C3, Apo E, Apo H, and Apo M proteins.\u003cbr\u003e\nHuman Lipoprotein A ELISA Kit ab108878 protocol summary:\u003cbr\u003e\n1. Add samples and standards to wells. Incubate at room temperature.\u003cbr\u003e\n2. Wash each well and add biotin-antibody conjugate. Incubate at room temperature.\u003cbr\u003e\n3. Wash each well and add Streptavidin Solution. Incubate at room temperature.\u003cbr\u003e\n4. Add Chromogen Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read immediately.\u003cbr\u003e\nHow other researchers are using Human Lipoprotein A ELISA Kit ab108878\u003cbr\u003e\nHuman Lipoprotein A ELISA Kit ab108878 has been used to measure Lipoprotein A in the blood as high levels correlate to increased risk factor for myocardial infarction (MI), coronary heart isease (CHD)\u003cbr\u003e\n, cerebrovascular disease (CVD), atherosclerosis, thrombosis, and stroke, and to measure serum biomarkers to determine the effect of compounds on reducing coronary heart disease\u003cbr\u003e\nReferences:\u003cbr\u003e\nM. Sanlialp, et.al., 2020, PMID: 31788836\u003cbr\u003e\nB. Liu, et.al., 2016, PMID: 27366196\u003cbr\u003e\nGet results in 90 minutes with our SimpleStep ELISA\u003cbr\u003e\n- Human Lipoprotein A ELISA Kit\u003cbr\u003e\nab212165\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843567079593,"sku":"ab108878","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab108878","provider":"Iright","version":"1.0","type":"link"}