{"product_id":"abcam-ab109235","title":"Abcam, ab109235, Anti-Xanthine Oxidase antibody [EPR4605]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Xanthine Oxidase antibody [EPR4605] (ab109235) is a rabbit monoclonal antibody detecting Xanthine Oxidase in  Western Blot, IHC-P . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR4605,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Xanthine Oxidase antibody [EPR4605] (ab109235) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of Xanthine Oxidase?\u003cbr\u003e\nAnti-Xanthine Oxidase [EPR4605] (ab109235) specifically detects a band for Xanthine Oxidase (UniProt: P47989) at a molecular weight of 146kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Xanthine Oxidase [EPR4605] (ab109235) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR4605] also available for your convenience: ab109235, Carrier free -\u003cbr\u003e\nab246338\u003cbr\u003e\n, PE -\u003cbr\u003e\nab317895\u003cbr\u003e\n, APC -\u003cbr\u003e\nab317933\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab317973\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab318014\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab318056\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab318098\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab320939\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nXanthine oxidase also known as XO is an enzyme often referred to as xanthine dehydrogenase\/oxidase. It has an important role in purine metabolism converting hypoxanthine to xanthine and then xanthine to uric acid. This enzyme is an aldehyde oxidase and belongs to the family of molybdenum-containing enzymes. Xanthine oxidase has a molecular weight of approximately 290 kDa. It is expressed in various tissues predominantly in the liver and small intestine with lower levels in kidney and lung tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nXanthine oxidase has a significant role in the production of reactive oxygen species such as superoxide during the process of purine catabolism. In its dehydrogenase form XO can interconvert into an oxidase form engaging more readily in the electron transfer that leads to reactive oxygen species generation. Xanthine oxidase is not a part of any larger complex but functions as a dimer with two identical subunits that facilitate its metabolic roles.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe enzyme is pivotal in the purine degradation pathway which is essential for the breakdown and eventual excretion of purine compounds as uric acid. This pathway connects it to enzymes like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which performs salvage reactions to recycle purines. Additionally xanthine oxidase's role in reactive oxygen species production links it to oxidative stress pathways impacting proteins such as superoxide dismutase which works to detoxify reactive intermediates.\u003cbr\u003e\nXanthine oxidase is closely implicated in conditions like gout and hyperuricemia due to its role in uric acid production. Elevated activity of XO can lead to excessive uric acid accumulation contributing to these disorders. Furthermore XO is associated with cardiovascular diseases as the generated reactive oxygen species can foster endothelial dysfunction. The enzyme's activity is intertwined with systems involving proteins like endothelial nitric oxide synthase where oxidative stress affects nitric oxide availability and vascular health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848550731945,"sku":"ab109235","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab109235","provider":"Iright","version":"1.0","type":"link"}