{"product_id":"abcam-ab109715","title":"Abcam, ab109715, ATP Synthase Immunocapture Kit","description":"\u003cp\u003eSize: 250µg\u003cbr\u003e\nab109715 ATP Synthase Immunocapture Kit allows isolation of ATP Synthase complex from small amounts of tissue.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Rat, Cow, Human,\u003cbr\u003e\nAssay type:Quantitative\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\n250 μg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 μg of ATP synthase (Complex V) from heart mitochondria.\u003cbr\u003e\nab109715 allows isolation of the ATP synthase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for ab109715 include but are not limited to examining alterations of ATP synthase subunits in inherited mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension and aging.\u003cbr\u003e\nNote: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (\u003cbr\u003e\nab109857\u003cbr\u003e\n\/MS910).\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nATP synthase also known as Complex V or F1F0-ATPase catalyzes the synthesis of ATP from ADP and inorganic phosphate. The multisubunit enzyme has a mass of approximately 500 kDa and resides in the inner mitochondrial membrane. It also appears in bacterial plasma membranes and the thylakoid membrane of chloroplasts. ATP synthase operates through a rotary mechanism converting a proton gradient into mechanical energy which then converts into chemical energy in the form of ATP.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nATP synthase functions as a critical component of cellular energy production. As part of the ATP synthase complex it carries out ATP synthesis essential for numerous biological processes. By utilizing a proton-motive force it enables the regeneration of mitochondrial ATP integral for maintaining cellular energy homeostasis. The enzyme's efficient ATP activity highlights its role in ensuring sustained energy supply within cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nATP synthase plays an important role in the oxidative phosphorylation pathway. This pathway occurs in mitochondria where the enzyme functions alongside other proteins such as NADH dehydrogenase and cytochrome c oxidase. These proteins forming the electron transport chain contribute to establishing the proton gradient required for ATP synthase activity. Additionally ATP synthase activity is closely linked to the citric acid cycle providing the necessary gradient for ATP production.\u003cbr\u003e\nATP synthase relates to conditions such as mitochondrial diseases and neurodegenerative disorders. Dysfunctional mitochondrial ATP synthase can lead to energy deficiency and mitochondrial myopathies. It also has connections to proteins like mitochondrial DNA mutations which impact ATP synthase function and cause cellular energy deficits. Furthermore impaired ATP synthase activity can contribute to conditions like Parkinson's disease where energy metabolism disruptions play a role.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843624718505,"sku":"ab109715","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab109715","provider":"Iright","version":"1.0","type":"link"}