{"product_id":"abcam-ab109800","title":"Abcam, ab109800, Complex III Immunocapture Kit","description":"\u003cp\u003eSize: 250µg\u003cbr\u003e\nab109800 Complex III Immunocapture Kit allows isolation of ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Mouse, Rat, Cow, Human,\u003cbr\u003e\nAssay type:Quantitative\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\n250 μg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 μg of Complex III from heart mitochondria.\u003cbr\u003e\nab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.\u003cbr\u003e\nNote: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (\u003cbr\u003e\nab109857\u003cbr\u003e\n\/MS910).\u003cbr\u003e\nRelated products\u003cbr\u003e\nReview the\u003cbr\u003e\nmitochondrial assay guide\u003cbr\u003e\n, or the full\u003cbr\u003e\nmetabolism assay guide\u003cbr\u003e\nto learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nComplex III also known as cytochrome c reductase or cytochrome bc1 complex plays an important role in the mitochondrial electron transport chain. This multiprotein complex weighing approximately 250 kDa facilitates electron transfer from ubiquinol to cytochrome c. Complex III resides in the inner mitochondrial membrane of eukaryotic cells and is important for cellular energy production. Structurally it consists of 11 subunits in mammals with cytochrome b cytochrome c1 and Rieske iron-sulfur protein being the core components.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nComplex III transfers electrons and contributes to proton translocation across the mitochondrial membrane. This process generates the proton gradient needed for ATP synthesis via oxidative phosphorylation often referred to as OXPHOS. As a core component of the mitochondrial respiratory chain Complex III interacts closely with other complexes mainly Complexes I and IV to maintain efficient energy conversion.\u003cbr\u003e\nPathways\u003cbr\u003e\nComplex III is integral to the oxidative phosphorylation pathway and the respiratory electron transport chain. It ensures efficient electron transport working closely with Complexes I II and IV. Cytochrome c acts as an electron carrier between Complex III and Complex IV in this pathway. This coordinated interaction is essential for ATP generation meeting cellular energy demands.\u003cbr\u003e\nComplex III is linked with mitochondrial disorders and neurodegenerative diseases. Deficiencies or mutations in its components may lead to disorders such as mitochondrial encephalopathy or Leigh syndrome which are associated with impaired energy metabolism. The complex's dysfunction also affects proteins connected to these diseases like ATP synthase due to disrupted energy production. Additionally its role in oxidative stress has implications for conditions like mitotox-related mitochondrial damage.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843629764777,"sku":"ab109800","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab109800","provider":"Iright","version":"1.0","type":"link"}