{"product_id":"abcam-ab112119","title":"Abcam, ab112119, Resazurin Assay Kit - Cell Cytotoxicity (Fluorometric)","description":"\u003cp\u003eSize: 1000Test \/ 5000Tests\u003cbr\u003e\nRecommended alternative kitWe recommend that you purchase Resazurin Assay Kit ab129732 instead of this kit.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nRecommended alternative kit\u003cbr\u003e\nWe recommend that you purchase Resazurin Assay Kit\u003cbr\u003e\nab129732\u003cbr\u003e\ninstead of this kit.\u003cbr\u003e\nResazurin Assay Kit ab112119 (Cell Cytotoxicity, Fluorometric) ab112119 provides a fast, simple, accurate and homogeneous assay for the fluorometric detection of viable cells.\u003cbr\u003e\nThe resazurin assay protocol is based on the reduction of oxidized non-fluorescent blue resazurin to a red fluorescent dye (resorufin) by the mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells. Resorufin has Ex\/Em of 530-560 \/ 590 nm.\u003cbr\u003e\nThe resazurin assay is very commonly used to quantify the numbers of live cells, and monitor cell viability \/ cytotoxicity.\u003cbr\u003e\nab112119 is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are stable and have minimal cytotoxicity, thus a longer incubation time (such as 24-48 hours) is possible if required.\u003cbr\u003e\nResazurin assay protocol summary:\u003cbr\u003e\n- add assay solution to cells in 96 or 384 well plates and shake gently for 30 sec\u003cbr\u003e\n- incubate for 1-24 hr at 37°C\u003cbr\u003e\n- analyze with microplate reader\u003cbr\u003e\nab112119 should be stored desiccated\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855602602153,"sku":"ab112119","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab112119","provider":"Iright","version":"1.0","type":"link"}