{"product_id":"abcam-ab112155","title":"Abcam, ab112155, Pyrophosphate Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 2 x 96Tests\u003cbr\u003e\nPyrophosphate Assay Kit (Fluorometric) ab112155 provides the most robust spectrophotometric method for the measurement of pyrophosphate.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Tissue Lysate, Urine, Plasma, Cell culture extracts, Suspension cells, Serum, Adherent cells,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPyrophosphate Assay Kit (Fluorometric) ab112155 provides the most robust spectrophotometric method for the measurement of pyrophosphate.\u003cbr\u003e\nThe pyrophosphate assay protocol uses a proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate.\u003cbr\u003e\nThis pyrophosphate assay protocol is much easier and more robust than enzyme-coupling pyrophosphate methods, which require at least two enzymes for their pyrophosphate detections. Due to its direct measurement of pyrophosphate, this kit is ideal for screening inhibition or activities of enzymes that consume or generate pyrophosphate.\u003cbr\u003e\nThe pyrophosphate assay is an optimized mix-and-read assay and can be performed in a convenient 96-well or 384-well microtiter-plate format.\u003cbr\u003e\nAssay time:\u003cbr\u003e\n10-30 minutes\u003cbr\u003e\nThis product measures PPi formation at Ex\/Em = 316\/456 nm.\u003cbr\u003e\nIf your instrument has filters for Ex\/Em = 370\/470 nm, we recommend using Pyrophosphate Assay Kit II (Fluorometric) (\u003cbr\u003e\nab179836\u003cbr\u003e\nOther Notes\u003cbr\u003e\nPyrophosphates (PPi) are produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.\u003cbr\u003e\nSamples such as plasma and serum might need a deproteinization step with spin columns\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInorganic pyrophosphate often abbreviated as PPi acts as a byproduct of several biosynthetic reactions. It possesses a molecular mass of 177.98 g\/mol. Pyrophosphate functions as an energy-rich intermediate that participates actively in reactions like the synthesis of nucleic acids and proteins. Inorganic pyrophosphatase an enzyme frequently associated with pyrophosphate metabolism catalyzes the hydrolysis of PPi releasing inorganic phosphate. This hydrolysis occurs release energy facilitating various biochemical processes. Pyrophosphate molecules are detected in tissues with high cell proliferation such as liver kidney and bones.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nInorganic pyrophosphate plays a critical part in bone mineralization and the regulation of calcium and phosphate metabolism. It influences the bone matrix's deposition by calcifying or inhibiting mineralization depending on the balance with phosphates. Within the extracellular matrix pyrophosphate remains associated with proteins such as alkaline phosphatase which converts PPi to phosphate promoting mineralization. Pyrophosphate's activity shows strong relevance in maintaining extracellular fluid stability preventing excessive calcification.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of inorganic pyrophosphate occurs prominently in the regulation of the ATP metabolism pathway and cartilage formation. It is closely related to the enzyme family of pyrophosphatases which include both soluble inorganic pyrophosphatase and membrane-integrated soluble pyrophosphatases. These enzymes orchestrate converting pyrophosphate into orthophosphate ions feeding into the pathways that regulate energy homeostasis and biomineralization. Interaction with proteins like ENPP1 and TNAP highlight its role within the broader context of nucleic acid synthesis and tissue calcification.\u003cbr\u003e\nAbnormal levels of inorganic pyrophosphate link to skeletal disorders like osteoporosis and osteoarthritis. High pyrophosphate concentrations can lead to pathological calcification processes prominently affecting joint health and bone density. The enzyme ectonucleotide pyrophosphatase\/phosphodiesterase 1 (ENPP1) connects to pyrophosphate's regulation of these diseases where it influences extracellular pyrophosphate concentration and subsequently mineralization. Understanding pyrophosphate dynamics provides insights into the pathophysiology of metabolic bone diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843559116969,"sku":"ab112155","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab112155","provider":"Iright","version":"1.0","type":"link"}