{"product_id":"abcam-ab113467","title":"Abcam, ab113467, DNMT Activity Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 48Test \/ 96Test\u003cbr\u003e\nDNMT Activity Assay Kit (Colorimetric) ab113467 allows the user to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-strip well microplate.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay time:3h 45m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nDNMT Activity Assay Kit (Colorimetric) ab113467 allows the user to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-strip well microplate.\u003cbr\u003e\nThe kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity\/inhibition experiment without the need for radioactivity or any special equipment.\u003cbr\u003e\nDNMT assay protocol method\u003cbr\u003e\nIn the DNMT assay protocol, DNMT enzymes in a sample transfer methyl groups from Adomet to a DNMT DNA substrate coated onto microplate wells. The methylated DNA is bound with a 5-methylcytosine antibody. A secondary antibody binds the 5-methylcytosine antibody, and the enzyme-conjugated secondary antibody is detected using a chromogenic enzyme substrate.\u003cbr\u003e\nDNMT assay protocol summary\u003cbr\u003e\n- add samples, positive controls, and samples with inhibitor to wells\u003cbr\u003e\n- incubate for 90-120 min, and wash wells three times with wash buffer\u003cbr\u003e\n- add capture antibody, incubate for 60 min, and wash wells\u003cbr\u003e\n- add detection antibody, incubate for 30 min, and wash wells\u003cbr\u003e\n- add enhancer solution, incubate for 30 min, and wash wells\u003cbr\u003e\n- add developer solution, incubate for 1-10 min\u003cbr\u003e\n- add stop solution and analyze with a microplate reader\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called DNMT Activity Quantification Kit (Colorimetric).\u003cbr\u003e\nDNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843545125033,"sku":"ab113467","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab113467","provider":"Iright","version":"1.0","type":"link"}