{"product_id":"abcam-ab115348","title":"Abcam, ab115348, Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts,\u003cbr\u003e\nReacts with:Mouse, Rat, Cow, Dog, Human,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nRange:60 - 250 µg\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample. The enzyme is captured within the wells of the microplate and activity is determined by following the production of NADH in the following ALDH2 catalyzed reaction:\u003cbr\u003e\nacetaldehyde + NAD\u003cbr\u003e\n-\u0026gt; acid + NADH\u003cbr\u003e\nThe generation of NADH in the aldehyde dehydrogenase assay protocol is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient - 37000 M\u003cbr\u003e\n). ab115348 immunocaptures in each well only native ALDH2 from the chosen sample; this removes all other enzymes, including unrelated aldehyde dehydrogenases.\u003cbr\u003e\nReagent dye, Coupler and NAD+ are shipped lyophilized. Before use rehydrate by adding 0.25 mL pure water to each tube and vortex each tube thoroughly to dissolve. After hydration unused amounts of these three materials should be stored at -80°C. Store all other components store at 4°C.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAldehyde Dehydrogenase (ALDH) an important enzyme responsible for oxidizing aldehydes into carboxylic acids participates in detoxifying aldehydes produced during various metabolic processes. ALDH has several isoforms including the well-studied ALDH2 which possesses a molecular mass of approximately 56 kDa. ALDH2 is found in many tissues but highest levels are in liver and kidney playing a significant role in processing endogenous aldehydes and those derived from external sources such as alcohol metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAldehyde Dehydrogenase influences the metabolism of aldehydrates aiding in the protection of cells from aldehyde-induced stress. ALDH exists within a larger family of enzymes and does not typically operate as part of a large complex. It helps maintain the balance of reactive aldehyde species in the cell through oxidation facilitating cellular protection against oxidative damage.\u003cbr\u003e\nPathways\u003cbr\u003e\nAldehyde Dehydrogenase plays an integral part in alcohol metabolism and the larger detoxification pathways. The enzyme ALDH2 is notably involved in the ethanol degradation pathway where it metabolizes acetaldehyde to acetate a less toxic compound. It interacts closely with alcohol dehydrogenase which initially forms acetaldehyde from ethanol establishing a sequential relationship within this critical metabolic pathway.\u003cbr\u003e\nALDH2's role is closely linked with alcoholism and Asian flush syndrome. ALDH2 deficiency impairs acetaldehyde detoxification leading to accumulation resulting in facial flushing and increased risk of alcoholism-related complications. Interaction with other proteins such as cytochrome P450 enzymes can impact the severity of these conditions as they are involved in overlapping detoxification pathways that handle a variety of substrates and drugs.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843599749289,"sku":"ab115348","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab115348","provider":"Iright","version":"1.0","type":"link"}