{"product_id":"abcam-ab117134","title":"Abcam, ab117134, Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit","description":"\u003cp\u003eSize: 24Test \/ 48Test \/ 96Test\u003cbr\u003e\nDNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in  5-methylcytosine (5-mC) .\u003cbr\u003e\nKey facts\u003cbr\u003e\nAssay time:3h,\u003cbr\u003e\nReacts with:Mouse, Human\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nDNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in\u003cbr\u003e\n5-methylcytosine (5-mC)\u003cbr\u003e\n. In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide,\u003cbr\u003e\n5-hydroxymethylcytosine (5-hmC)\u003cbr\u003e\n, has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.\u003cbr\u003e\nHydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134) enables the user to enrich\u003cbr\u003e\nhydroxymethated DNA\u003cbr\u003e\nusing an antibody specific to hydroxymethylcytosine (5-hmC) to immunoprecipitate hydroxymethylated genomic DNA\u003cbr\u003e\nhMeDIP\u003cbr\u003e\n. The enriched hydroxymethylated fractions can be used in various downstream applications including PCR (hMeDIP-PCR) and microarrays (hMeDIP-chip) for investigating gene-specific hydroxymethylation in cells or tissues.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847600525481,"sku":"ab117134","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab117134","provider":"Iright","version":"1.0","type":"link"}