{"product_id":"abcam-ab119505","title":"Abcam, ab119505, Human APRIL ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman APRIL ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human APRIL in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.4 ng\/mL,\u003cbr\u003e\nRange:0.78 - 50 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's APRIL Human\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human APRIL concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).\u003cbr\u003e\nAPRIL specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an APRIL biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of APRIL captured on the plate.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAPRIL also known as TNFSF13 is a member of the tumor necrosis factor (TNF) ligand superfamily. This protein has a mass of approximately 31 kDa and undergoes incorporation into different cellular processes. APRIL is mainly expressed in cells of the immune system such as T cells B cells and myeloid cells. The protein plays a critical role in modulating various biological activities including cell survival proliferation and apoptosis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAPRIL functions as a cytokine involved in immune regulation. The molecule acts independently or as part of a ligand-receptor interaction binding specifically to receptors like BCMA and TACI. This interaction influences B-cell maturation and differentiation driving immune responses. APRIL helps maintain homeostasis within the immune system ensuring appropriate immune activation and preventing overactive immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nAPRIL coordinates with several immune-related pathways notably the B-cell signaling pathway and the NF-kB signaling pathway. These pathways are essential in immune system modulation influencing how the body responds to pathogens. APRIL interacts with proteins such as BAFF (B-cell activating factor) which share common receptors shaping the immune response through these pathways and ensuring proper immune cell function.\u003cbr\u003e\nAPRIL has connections with conditions like autoimmune diseases and certain lymphomas. Elevated levels of APRIL often occur in diseases like lupus contributing to abnormal B-cell activity and autoantibody production. Additionally its involvement in lymphomas links to excessive proliferation of B cells. Proteins such as BAFF and their receptors play a significant role in these conditions highlighting APRIL's impact on disease mechanisms and potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855670595753,"sku":"ab119505","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab119505","provider":"Iright","version":"1.0","type":"link"}