{"product_id":"abcam-ab124673","title":"Abcam, ab124673, Anti-Werner's syndrome helicase WRN antibody [EPR6392]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) is a rabbit monoclonal antibody detecting Werner's syndrome helicase WRN in  Western Blot . Suitable for  Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR6392,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of Werner's syndrome helicase WRN?\u003cbr\u003e\nAnti-Werner's syndrome helicase WRN [EPR6392] (ab124673) specifically detects a band for Werner's syndrome helicase WRN (UniProt: Q14191) at a molecular weight of 162kDa.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies.\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) has been confirmed by Western blot testing in WRN Knockout HAP1 cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR6392] also available for your convenience: ab124673, Carrier free -\u003cbr\u003e\nab247955\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb\u003cbr\u003e\npatents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nWerner's syndrome helicase also known as WRN plays a mechanical role in DNA replication and repair. WRN is a 160 kDa protein containing both helicase and exonuclease activities making it unique among RecQ helicases. It unwinds DNA duplexes and resolves complex DNA structures that can arise during replication and repair processes. WRN is expressed in the nucleus of various cell types including fibroblasts where it maintains genome stability. This helicase is critical in processing DNA intermediates to prevent genomic instability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nWRN functions as a part of larger protein complexes that participate in DNA damage response and repair. It interacts with several proteins involved in the homologous recombination and base excision repair pathways. WRN's unique enzymatic activities enable it to stabilize and process DNA ends during replication fork collapse and double-strand break repair. By operating in concert with proteins such as RAD51 and replication protein A (RPA) WRN ensures accurate replication and repair preventing mutation accumulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nWRN plays a central role in the maintenance of genomic integrity through its involvement in DNA repair pathways and the aging process. It is integral to the process of homologous recombination where it works closely with the MRE11-RAD50-NBS1 (MRN) complex. Additionally WRN has a function in the DNA damage checkpoint pathway collaborating with proteins like ATM and ATR which help to sense damaged DNA and initiate repair processes. These pathways are important for maintaining DNA integrity and preventing cellular senescence.\u003cbr\u003e\nWRN is closely linked to Werner syndrome and cancer. Mutations in the WRN gene lead to Werner syndrome a rare disorder characterized by premature aging and cancer predisposition. WRN's interaction with tumor suppressor proteins such as p53 connects it to pathways that can contribute to tumorigenesis when dysregulated. Understanding WRN's function and its role in these diseases provides insight into mechanisms of aging and cancer development offering potential therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844196061353,"sku":"ab124673","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab124673","provider":"Iright","version":"1.0","type":"link"}