{"product_id":"abcam-ab128873","title":"Abcam, ab128873, Anti-CTCF antibody [EPR7314(B)] - ChIP Grade","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) is a rabbit monoclonal antibody detecting CTCF in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF, ChIP . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7314(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, IHC-P, ChIP, Flow Cyt (Intra), ChIC\/CUT\u0026amp;RUN-seq, WB, CUT\u0026amp;Tag, ChIP-seqSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF), ChIP in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of CTCF?\u003cbr\u003e\nAnti-CTCF [EPR7314(B)] - ChIP Grade (ab128873) specifically detects a band for CTCF (UniProt: P49711) at a molecular weight of 83kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-CTCF [EPR7314(B)] - ChIP Grade (ab128873) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR7314(B)] also available for your convenience: ab128873, Alexa Fluor® 488 -\u003cbr\u003e\nab203704\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab203705\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab240035\u003cbr\u003e\n, PE -\u003cbr\u003e\nab305764\u003cbr\u003e\n, APC -\u003cbr\u003e\nab305765\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab305766\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308816\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310547\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab312077\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312554\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321018\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nShipping conditions update: ambient shipping\u003cbr\u003e\nThis product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.\u003cbr\u003e\nWhy the change?\u003cbr\u003e\nIt’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.\u003cbr\u003e\nWhat you need to know\u003cbr\u003e\nAmbient shipments come clearly marked on the delivery note.\u003cbr\u003e\nNo ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.\u003cbr\u003e\nWarranty coverage remains fully valid, aligned with our validated shipping method.\u003cbr\u003e\nPlease store the product as per the datasheet instructions upon receipt.\u003cbr\u003e\nFind out more -\u003cbr\u003e\nhttps:\/\/www.abcam.com\/en-us\/support\/shipping-storage-support\/ambient-shipping\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.2% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCTCF also known as the CCCTC-binding factor is a highly conserved zinc finger protein involved in transcriptional regulation and chromatin organization. It has a molecular weight of approximately 82 kDa. The CTCF protein plays a critical mechanical role in controlling the three-dimensional architecture of the genome by binding to specific DNA sequences and forming chromatin loops. It is widely expressed in various cell types across tissues where it acts as a transcriptional repressor and activator depending on the context. CTCF immunofluorescence techniques enable the visualization of its dynamic distribution and expression within the nucleus.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe multipurpose CTCF protein acts in coordinating the spatial organization of the genome. It functions as an insulator by regulating the boundaries between different chromosomal domains and controlling gene expression. CTCF operates within various complexes interacting with cohesin a vital protein complex that facilitates loop formation and influences genome architecture. This interaction helps in maintaining the integrity of the genome structure and proper chromatin insulation which are essential for normal gene function.\u003cbr\u003e\nPathways\u003cbr\u003e\nCTCF plays significant roles in epigenetic regulatory networks and transcriptional pathways. In the epigenetic landscape it influences gene expression through modulation of DNA methylation states at CpG islands interacting with proteins like DNA methyltransferases. In transcriptional pathways CTCF interacts with nuclear factor Y (NF-Y) which contributes to cell cycle regulation by modulating the expression of cell cycle genes. These pathways reflect CTCF's versatility in gene regulation and its influence on maintaining cellular homeostasis.\u003cbr\u003e\nCTCF disruptions have been implicated in cancer and intellectual disabilities. Mutations or altered expression of CTCF can lead to tumorigenesis as CTCF acts as a tumor suppressor by controlling oncogene and tumor suppressor gene expression. In intellectual disabilities CTCF mutations affect brain development by disrupting the expression of neuronal genes. The protein's interaction with cohesin has links to disorders such as Cornelia de Lange syndrome where cohesin complex dysfunction parallels the phenotypes seen with CTCF aberrations.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844055060649,"sku":"ab128873","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab128873","provider":"Iright","version":"1.0","type":"link"}