{"product_id":"abcam-ab128915","title":"Abcam, ab128915, Anti-GAPDH antibody [EPR6256] - Loading Control","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-GAPDH antibody [EPR6256] - Loading Control (ab128915) is a rabbit monoclonal antibody detecting GAPDH in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  African green monkey, Human . - Biophysical QC for unrivalled batch-batch consistency - Over 270 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR6256,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human, African green monkey,\u003cbr\u003e\nApplications:ICC\/IF, IHC-P, Flow Cyt (Intra), WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The African green monkey recommendation is based on the WB results. We do not guarantee IHC-P for African green monkey.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-GAPDH antibody [EPR6256] - Loading Control (ab128915) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in African green monkey, Human samples.\u003cbr\u003e\nWhat is the molecular weight of GAPDH?\u003cbr\u003e\nAnti-GAPDH [EPR6256] - Loading Control (ab128915) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P04406) at a molecular weight of 36kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-GAPDH [EPR6256] - Loading Control (ab128915) was first used in a scientific publication in 2012 and has been cited over 270 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-GAPDH [EPR6256] - Loading Control (ab128915) has over 10 independent reviews from customers.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR6256] also available for your convenience: ab128915, HRP -\u003cbr\u003e\nab185059\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab186930\u003cbr\u003e\n, Biotin -\u003cbr\u003e\nab195904\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab215227\u003cbr\u003e\n, PerCP -\u003cbr\u003e\nab216377\u003cbr\u003e\n, APC -\u003cbr\u003e\nab221270\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb\u003cbr\u003e\npatents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nShipping conditions update: ambient shipping\u003cbr\u003e\nThis product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.\u003cbr\u003e\nWhy the change?\u003cbr\u003e\nIt’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.\u003cbr\u003e\nWhat you need to know\u003cbr\u003e\nAmbient shipments come clearly marked on the delivery note.\u003cbr\u003e\nNo ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.\u003cbr\u003e\nWarranty coverage remains fully valid, aligned with our validated shipping method.\u003cbr\u003e\nPlease store the product as per the datasheet instructions upon receipt.\u003cbr\u003e\nFind out more -\u003cbr\u003e\nhttps:\/\/www.abcam.com\/en-us\/support\/shipping-storage-support\/ambient-shipping\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle|Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nGAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.\u003cbr\u003e\nGAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH's altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844033630377,"sku":"ab128915","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab128915","provider":"Iright","version":"1.0","type":"link"}