{"product_id":"abcam-ab131368","title":"Abcam, ab131368, Anti-mouse IgG for IP (HRP)","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRat anti-mouse IgG (gamma chain-specific) (HRP) (ab131368) a horseradish peroxidase-secondary antibody designed for sensitive chemiluminescent detection in Western blot, IP and ELISA applications. - Proven performance: cited in over 145 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rat,\u003cbr\u003e\nTarget species:Mouse,\u003cbr\u003e\nTarget isotype:IgG,\u003cbr\u003e\nTarget specificity:Gamma chain,\u003cbr\u003e\nPre-adsorbed:No,\u003cbr\u003e\nConjugation:HRP,\u003cbr\u003e\nApplications:WB, ELISA, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:eB144,\u003cbr\u003e\nIsotype:IgG\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nNumber of blots:\u003cbr\u003e\nAt least\u003cbr\u003e\n20 blots,\u003cbr\u003e\nbased on a 1 μl\/ml dilution and 5 ml diluted antibody per blot.\u003cbr\u003e\nImportant protocol notes:\u003cbr\u003e\n1. The anti-mouse IgG for IP secondary antibody (HRP) detects mouse IgG antibodies (subtypes: IgG1, IgG2a, IgG2b, IgG3).\u003cbr\u003e\n2. The anti-mouse IgG for IP secondary antibody (HRP) preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured forms.\u003cbr\u003e\n3. IP sample should be completely reduced\/denatured before loaded onto a western blot.\u003cbr\u003e\n4. Milk should be used as the blocking protein for the immunoblot.\u003cbr\u003e\nWestern blot and IP resources:\u003cbr\u003e\nWestern blot a beginner's guide\u003cbr\u003e\nIP protocol\u003cbr\u003e\nIP troubleshooting tips\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.16% Sodium phosphate, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847518933161,"sku":"ab131368","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab131368","provider":"Iright","version":"1.0","type":"link"}