{"product_id":"abcam-ab132844","title":"Abcam, ab132844, Recombinant Human Acid sphingomyelinase protein","description":"\u003cp\u003eSize: 2µg\u003cbr\u003e\nRecombinant Human Acid sphingomyelinase protein is a Human Full Length protein, in the 1 to 364 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.\u003cbr\u003e\nKey facts\u003cbr\u003e\nExpression system:Wheat germ,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:SDS-PAGE, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P17405,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAcid sphingomyelinase (ASMase) also known as sphingomyelin phosphodiesterase 1 or NP is an enzyme involved in sphingolipid metabolism. ASMase has a mass of approximately 75 kDa and appears in lysosomes where it converts sphingomyelin to ceramide and phosphorylcholine. This enzyme is important in maintaining cellular lipid balance and signaling. Expression of ASMase occurs in various tissues such as the liver spleen and brain.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nASMase plays a role in membrane microdomain composition through its involvement in ceramide production. It participates in generating ceramide-enriched platforms that facilitate the clustering of signaling molecules. Ceramide acts as a second messenger in multiple cellular processes including apoptosis proliferation and inflammation. ASMase operates in the lysosomal lipid degradation pathway and connects with other lysosomal enzymes to modulate lipid turnovers such as glucosylceramidase affecting downstream cellular functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nSphingolipid metabolism involves ASMase. This enzyme participates in the ceramide signaling pathway influencing apoptosis and stress responses. Related proteins in this pathway include casein kinase II which phosphorylates ASMase and cathepsin D involved in the lysosomal degradation process. ASMase activity alters ceramide levels impacting pro-apoptotic and pro-survival signals mediated by related proteins in the cell signaling network.\u003cbr\u003e\nASMase deficiency connects to Niemann-Pick disease types A and B characterized by lipid accumulation in lysosomes. Mutations in the ASMase gene lead to impaired enzyme function resulting in excessive sphingomyelin storage and cell damage. The disorder links ASMase to proteins such as hexa-beta-N-acetylglucosaminidase which is affected in other lysosomal storage disorders. Research shows that ASMase activity also influences cardiovascular diseases by regulating ceramide and cholesterol levels in atherosclerotic lesions connecting it to inflammatory pathways involving adhesion molecules and cytokines.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843819655337,"sku":"ab132844","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab132844","provider":"Iright","version":"1.0","type":"link"}