{"product_id":"abcam-ab133089","title":"Abcam, ab133089, Phospholipase A2 Activity Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nPhospholipase A2 Activity Assay Kit (ab133089) provides an accurate and convenient method for measurement of PLA2 activity. Readout on any colorimetric (405 or 414 nm) plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhospholipase A2 Activity Assay Kit (ab133089) provides an accurate and convenient method for measurement of PLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (e.g., bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5'-dithio-bis-(2-nitrobenzoic acid)).\u003cbr\u003e\nRelated PLA2 assay kits\u003cbr\u003e\nFor a fluorometric PLA2 assay use Phospholipase A2 Activity Assay Kit\u003cbr\u003e\nab273278\u003cbr\u003e\nTo detect cytosolic PLA2, use Cytosolic Phospholipase A2 Assay Kit\u003cbr\u003e\nab133090\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called Phospholipase A2 Assay Kit.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPhospholipase A2 (PLA2) is an enzyme that hydrolyzes the sn-2 ester bond of phospholipids releasing fatty acids and lysophospholipids. This process is essential for producing pro-inflammatory eicosanoids and other lipid mediators. Commonly known as PLA2 or phospholipase its molecular mass varies depending on its type ranging from 14 to 45 kDa. PLA2 is expressed in many tissues including the pancreas brain and immune cells highlighting its diverse role in different physiological contexts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPhospholipase A2 contributes to membrane remodeling and the release of arachidonic acid a precursor to prostaglandins and leukotrienes vital for inflammatory responses. It often functions as part of a complex with other proteins that regulate its activity ensuring precise lipid signaling and homeostasis. Understanding PLA2 function leads to insights into cell signaling and inflammatory regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nPhospholipase A2 plays a significant role in the arachidonic acid pathway and is involved in the production of eicosanoids. These pathways are important for the inflammatory response and involve interactions with cyclooxygenases (COX) and lipoxygenases (LOX). Through these pathways PLA2 activity can influence immune responses and cellular metabolism making it an important component in inflammatory signaling cascades.\u003cbr\u003e\nPhospholipase A2 is implicated in conditions like arthritis and atherosclerosis. Its role in inflammation makes it a potential target for therapeutic interventions. PLA2 interacts with proteins such as COX-2 which are involved in the inflammatory response in arthritis while its role in lipid metabolism links it to the formation of atherosclerotic plaques. Understanding these connections can aid in developing treatments for inflammatory and cardiovascular diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843629830313,"sku":"ab133089","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab133089","provider":"Iright","version":"1.0","type":"link"}