{"product_id":"abcam-ab133406","title":"Abcam, ab133406, ECL Substrate Kit (High Sensitivity)","description":"\u003cp\u003eSize: 20mL \/ 200mL \/ 500mL\u003cbr\u003e\nECL Substrate Kit (High Sensitivity) (ab133406) is an enhanced chemiluminescent substrate designed for the detection of proteins with 23 pg-187 ng of protein per band. - Long lasting, highly sensitivity substrate- strong signal with very low background - Use for film imaging and compatible with CCD imaging - Available in different sizes to fit your experimental needs\u003cbr\u003e\nProduct details:\u003cbr\u003e\nHigh Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.\u003cbr\u003e\nOur ECL kits include our popular ECL Substrate Kit\u003cbr\u003e\nab65623\u003cbr\u003e\nand our high sensitivity ECL substrate kits:\u003cbr\u003e\n- this kit (High Sensitivity ECL Substrate Kit ab133406) to detect 23pg-187ng of protein per band\u003cbr\u003e\n- Very High Sensitivity ECL Substrate Kit\u003cbr\u003e\nab133408\u003cbr\u003e\nto detect 4.6pg-4.7ng of protein per band\u003cbr\u003e\n- Ultra High Sensitivity ECL Substrate Kit\u003cbr\u003e\nab133409\u003cbr\u003e\nto detect 1.2pg-2ng of protein per band\u003cbr\u003e\nDetection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.\u003cbr\u003e\nThis product was previously called Optiblot ECL Detect Kit (23pg-187ng).\u003cbr\u003e\n- 200ml kit for 2000cm\u003cbr\u003e\nmembrane\u003cbr\u003e\n- 500ml kit for 5000cm\u003cbr\u003e\nmembrane\u003cbr\u003e\nThe primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1\/5000 - 1\/20,000, with a typical secondary antibody dilution range of 1\/20,000 - 1\/100,000. Some optimisation may be required.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843539685545,"sku":"ab133406","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab133406","provider":"Iright","version":"1.0","type":"link"}