{"product_id":"abcam-ab133627","title":"Abcam, ab133627, Anti-MyoD1 antibody [EPR6653-131]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-MyoD1 antibody [EPR6653-131] (ab133627) is a rabbit monoclonal antibody detecting MyoD1 in  Western Blot, IHC-P, ICC\/IF . Suitable for  Human . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR6653-131,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ChIC\/CUT\u0026amp;RUN-seq, IHC-P, WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-MyoD1 antibody [EPR6653-131] (ab133627) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of MyoD1?\u003cbr\u003e\nAnti-MyoD1 [EPR6653-131] (ab133627) specifically detects a band for MyoD1 (UniProt: P15172) at a molecular weight of 34kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-MyoD1 [EPR6653-131] (ab133627) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-MyoD1 [EPR6653-131] (ab133627) has over 10 independent reviews from customers.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR6653-131] also available for your convenience: ab133627, Carrier free -\u003cbr\u003e\nab240073\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab311001\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab311127\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab311759\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab313039\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab313240\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321061\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMyoD1 also known as Myogenic Differentiation 1 is a master regulator of muscle differentiation. It functions mechanically as a transcription factor binding to DNA at specific sequences thereby activating the transcription of genes necessary for muscle tissue development. MyoD1 has a molecular weight of approximately 45 kDa. It is expressed in skeletal muscle tissues and also detected in some non-muscle tissues though at lower levels. MyoD1 is essential in the early stages of muscle cell lineage commitment.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMyoD1 plays an important role in muscle differentiation by activating muscle-specific genes. It belongs to the myogenic regulatory factor family and often operates within a protein complex alongside Myf5 and myogenin aiding in the conversion of mesodermal stem cells into muscle cells. These partnerships enhance its ability to initiate the muscle-specific gene expression that drives myogenesis. MyoD1 acts like a molecular switch shifting cells into the pathway leading to muscle formation.\u003cbr\u003e\nPathways\u003cbr\u003e\nMyoD1 is an integral component of myogenesis and muscle regeneration pathways. MyoD1 interacts with key pathway components such as Myf5 and MRF4 which reinforce MyoD1 activity in muscle tissue lineage determination and cell cycle arrest during differentiation. The activity of MyoD1 in these pathways highlights its role in skeletal muscle growth. MyoD1 also demonstrates functional redundancy with Myogenin as both share overlapping roles in these critical pathways.\u003cbr\u003e\nMyoD1 is associated with muscular dystrophy and some sarcomas. Aberrant expression or mutations in MyoD1 can disrupt normal muscle differentiation contributing to muscle-related disorders like rhabdomyosarcoma a form of cancer comprising cells resembling skeletal muscle. Its relationship with other proteins like Myf5 further highlights its role in such pathological conditions as faults in these connections can amplify or alter disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844142583977,"sku":"ab133627","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab133627","provider":"Iright","version":"1.0","type":"link"}