{"product_id":"abcam-ab134845","title":"Abcam, ab134845, Recombinant Bacteriophage P1 Cre recombinase protein","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nRecombinant Bacteriophage P1 Cre recombinase protein is a Bacteriophage P1 Full Length protein, in the 2 to 343 aa range, expressed in Escherichia coli, with \u0026gt;90%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P06956,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Bacteriophage P1,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.08% Potassium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nApplications:\u003cbr\u003e\n1. Protein intracellular transduction for in vitro Cre-loxP recombination.\u003cbr\u003e\n2. Negative control for 11R-Tag human transcription factor mediated cell differentiation assay in vitro.\u003cbr\u003e\nFunctional Assay: Mix 1μg Cre-11R protein with 2ul ProFectinTM by following ProFectinTM manufacture suggested protocol, and incubate transfection mixture  0.25 x10\u003cbr\u003e\n293-LoxP-eGFP cell line \/ well (6 well plate) for 30 min in serum free medium, will achieve \u0026gt; 35% transduction efficiency. (Positive for GFP cells).\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843999879337,"sku":"ab134845","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab134845","provider":"Iright","version":"1.0","type":"link"}