{"product_id":"abcam-ab13575","title":"Abcam, ab13575, Anti-Cytochrome C antibody [7H8.2C12]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-Cytochrome C antibody [7H8.2C12] (ab13575) is a mouse monoclonal antibody detecting Cytochrome C in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human . - Over 200 publications - Trusted since 2004\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:7H8.2C12,\u003cbr\u003e\nIsotype:IgG2b,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, Flow Cyt (Intra), ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nEpitope:The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, based on competitive ELISA results.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Cytochrome C antibody [7H8.2C12] (ab13575) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of Cytochrome C?\u003cbr\u003e\nAnti-Cytochrome C [7H8.2C12] (ab13575) specifically detects a band for Cytochrome C (UniProt: P99999) at a molecular weight of 12kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Cytochrome C [7H8.2C12] (ab13575) was first used in a scientific publication in 2004 and has been cited over 200 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-Cytochrome C [7H8.2C12] (ab13575) has over 30 independent reviews from customers.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [7H8.2C12] also available for your convenience: ab13575, HRP -\u003cbr\u003e\nab199222\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab237966\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCytochrome C also referred to as \"cytochrome c\" or \"Cyt C\" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.\u003cbr\u003e\nPathways\u003cbr\u003e\nCytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.\u003cbr\u003e\nCytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855748059305,"sku":"ab13575","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab13575","provider":"Iright","version":"1.0","type":"link"}