{"product_id":"abcam-ab136721","title":"Abcam, ab136721, Rabbit Anti-Sheep IgG H\u0026L (Glucose Oxidase)","description":"\u003cp\u003eSize: 500µL\u003cbr\u003e\nSuitable for IHC-Fr, IHC-P, ICC, ELISA, WB.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nTarget species:Sheep,\u003cbr\u003e\nTarget isotype:IgG,\u003cbr\u003e\nTarget specificity:Heavy \u0026amp; Light chains,\u003cbr\u003e\nPre-adsorbed:No,\u003cbr\u003e\nConjugation:Glucose Oxidase,\u003cbr\u003e\nApplications:IHC-P, WB, IHC-Fr, ICC, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nDo not store in plastic tubes (i.e. eppendorf) to avoid losing activity.\u003cbr\u003e\nPreparation of Substrate Solutions:\u003cbr\u003e\nELISA: 10mg\/ml D(+)glucose, 0.1mg\/ml grade II (Rz=0.6) peroxidase, 0.35mg\/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V\/2 volume of 2N H\u003cbr\u003e\n. Reading at 492 nm.\u003cbr\u003e\nImmunocytochemistry: 15mg\/ml D(+)glucose, 0.5mg\/ml blue thiazolyl, 0.35mg\/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab136721 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography)., Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847553798313,"sku":"ab136721","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab136721","provider":"Iright","version":"1.0","type":"link"}