{"product_id":"abcam-ab136735","title":"Abcam, ab136735, Rabbit F(ab) Anti-Pig IgG H\u0026L (HRP)","description":"\u003cp\u003eSize: 500µL\u003cbr\u003e\nSuitable for WB, ELISA, IHC-Fr, IHC-P, ICC. Ideal for western blot. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nTarget species:Pig,\u003cbr\u003e\nTarget isotype:IgG,\u003cbr\u003e\nTarget specificity:Heavy \u0026amp; Light chains,\u003cbr\u003e\nPre-adsorbed:No,\u003cbr\u003e\nConjugation:HRP,\u003cbr\u003e\nApplications:IHC-Fr, WB, ELISA, IHC-P, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPreparation of Substrate Solutions:\u003cbr\u003e\nELISA: OPD (orthophenylene diamine): 0.4 mg\/ml in a 0.1M pH5 phosphate citrate buffer + 0.4 µl\/ml H\u003cbr\u003e\n(30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V\/2 volume of 1M H\u003cbr\u003e\n. Reading at 492nm\u003cbr\u003e\nImmunocytochemistry: DAB (diamino-benzidine): 0.5 mg\/ml in a 0.1M pH7.4 TRIS\/HCl buffer + 5 µl\/ml H\u003cbr\u003e\n(30% hydrogen peroxide solution). Incubation 10 minutes in darkness.\u003cbr\u003e\nWestern Blot: 4CN (4-chloro-1naphtol): 25 mg in 49.5 ml of 0.1M pH 7.4 PBS + 5 µl\/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H\u003cbr\u003e\n. Incubate 10 minutes at room temperature.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Constituents: 50% Glycerol (glycerin, glycerine), 49% Tris buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847546130601,"sku":"ab136735","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab136735","provider":"Iright","version":"1.0","type":"link"}