{"product_id":"abcam-ab136764","title":"Abcam, ab136764, Rabbit F(ab')2 Anti-Dog IgG H\u0026L (Alkaline Phosphatase)","description":"\u003cp\u003eSize: 500µL\u003cbr\u003e\nSuitable for FuncS, ELISA, IHC-P, WB, IHC-Fr, ICC.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nTarget species:Dog,\u003cbr\u003e\nTarget isotype:IgG,\u003cbr\u003e\nTarget specificity:Heavy \u0026amp; Light chains,\u003cbr\u003e\nPre-adsorbed:No,\u003cbr\u003e\nConjugation:Alkaline Phosphatase,\u003cbr\u003e\nApplications:IHC-Fr, FuncS, ICC, IHC-P, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPreparation of Substrate Solutions:\u003cbr\u003e\nELISA : 1M diethanolamin, 0.5mM MgCl\u003cbr\u003e\n, 15mM pNPP, pH 9.8. Incubate for 15 minutes at 37°C. Stop the reaction with V\/5 K\u003cbr\u003e\n2M. Read at 405 nm.\u003cbr\u003e\nImmunoblot : 34 µl of BCIP (5bromo-4chloroindolylphosphate) at 50mg\/ml in DMF + 44 µl NBT (NitroBlue Tetrazolium) at 75 mg\/ml in DMF 70% + 10ml substrate buffer (0.1M TRIS\/HCI, 0.5M NaCl, 0.05M MgCl\u003cbr\u003e\n, pH 9.5).\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab136764 is purified by affinity chromatography and is thus particularly specific., Storage buffer-Preservative: 0.1% Sodium azideConstituents: 50% Glycerol (glycerin, glycerine), 48.9% Tris buffered saline, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847552389289,"sku":"ab136764","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab136764","provider":"Iright","version":"1.0","type":"link"}