{"product_id":"abcam-ab136810","title":"Abcam, ab136810, Cell Cycle (pCdk\/pHH3\/Actin) WB Cocktail","description":"\u003cp\u003eSize: 200µL\u003cbr\u003e\nCell Cycle (pCdk\/pHH3\/Actin) WB Cocktail (ab136810) is part of the reagents, controls \u0026amp; accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Preservative: 0.01% Sodium azideConstituents: 40% Glycerol (glycerin, glycerine), 0.7% Sodium chloride, 0.61% Tris, 0.05% BSA\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe Cell Cycle western blot cocktail (ab136810) is designed to provide a rapid assessment of cell cycle distribution based on molecular markers for the G1\/S and M phases of the cell cycle. This is a mixture of three specific rabbit monoclonal primary antibodies targeting phospho-cdk2 Tyr15, phospho Histone H3 Ser10 and beta-actin. Cyclin-dependent kinase 2 (Cdk2) is maintained in an inactive state in G1\/S by inhibitory phosphorylation on Tyr15. Histone H3 is phosphorylated at Ser10 when chromosomes condense during mitosis. Hence, elevated phospho-cdk2 Tyr15 indicates G1\/S arrested cells and elevated phospho Histone H3 Ser10 indicates M-phase arrested cells when compared to asynchronous cycling control cells. An anti- Actin antibody is included as a loading control. These three readouts are easily resolved by western blot given their different molecular weights.\u003cbr\u003e\nThe recommended dilution for this cocktail is 1:250.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847572967593,"sku":"ab136810","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab136810","provider":"Iright","version":"1.0","type":"link"}