{"product_id":"abcam-ab136955","title":"Abcam, ab136955, Glucose Uptake Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nGlucose Uptake Assay Kit ab136955 is a simple and direct colorimetric assay for the measurement of glucose uptake in cell cultures. - Simple, non-radioactive protocol based on 2-deoxyglucose - Cited in over 180 publications - Suitable for both suspension \u0026amp; adherent cells - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt;= 0.01 nmol\/well,\u003cbr\u003e\nAssay time:3h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nGlucose Uptake Assay Kit (Colorimetric) is an assay that uses the glucose analog 2-deoxyglucose (2-DG) to detect and quantify glycose uptake in cells. 2-deoxyglucose (2-DG) is used in glucose uptake assay protocol because of its structural similarity to glucose. 2-DG is taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P cannot be further metabolized, and thus accumulates within cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In this assay, the 2-DG6P is oxidized to generate NADPH, the level of which can be determined by an enzymatic recycling amplification reaction.\u003cbr\u003e\nGlucose uptake assay protocol summary:\u003cbr\u003e\n- Prepare cells with suitable glucose starvation \/ uptake stimulation depending on experimental set-up\u003cbr\u003e\n- Add 2-DG to cells and incubate for 20 mins at 37°C\u003cbr\u003e\n- Wash cells with PBS to remove exogenous 2-DG\u003cbr\u003e\n- Lyse cells with extraction buffer and repeated pipetting\u003cbr\u003e\n- Freeze\/thaw lysates and heat at 85°C for 40 min\u003cbr\u003e\n- Cool on ice for 5 min\u003cbr\u003e\n- Add neutralizing buffer, spin and transfer supernatant to new tubes\u003cbr\u003e\n- Add supernatants and standards to wells\u003cbr\u003e\n- Add reaction mix A and incubate for 1 hr at 37°C\u003cbr\u003e\n- Add extraction buffer and heat to 90°C for 40 min\u003cbr\u003e\n- Cool on ice for 5 min and add neutralizing buffer\u003cbr\u003e\n- Add reaction mix B\u003cbr\u003e\n- Analyze every 2-3 mins on microplate reader in kinetic mode at 37°C\u003cbr\u003e\nHow other researchers are using Glucose Uptake Assay ab136955\u003cbr\u003e\nGlucose Uptake Assay Kit (Fluorometric) has been used in a variety of sample types including:\u003cbr\u003e\nHuman cervical epithelium HeLa and Human neuroblastoma SH-SY5Y\u003cbr\u003e\nHuman osteosarcoma cancer cell lines 143B and U2OS\u003cbr\u003e\nHuman breast cancer cell lines\u003cbr\u003e\nReferences:\u003cbr\u003e\n1-Son S et al. 2023\u003cbr\u003e\n2-Mei Z al. 2024\u003cbr\u003e\n3-Peng Z et al. 2024\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nFor fluorometric detection, we recommend Glucose Uptake Assay Kit (Fluorometric)\u003cbr\u003e\nab136956\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlucose uptake often referred to as \"glucose transport\" or \"glucose direct\" is the process by which cells absorb glucose from the bloodstream. This process requires specific transporter proteins primarily the glucose transporter family known as GLUTs. GLUTs differ in their affinities and tissue distributions; for example GLUT4 found in adipose tissues and muscle is insulin-sensitive. Glucose uptake mainly occurs in tissues with high energy demands like muscles and the brain ensuring these tissues receive adequate energy for function and development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe role of glucose uptake in cellular energy metabolism is paramount. It supplies glucose as a primary energy substrate especially in tissues that depend heavily on glycolysis. In cells glucose uptake does not act alone but is part of a complex metabolic carbohydrate mechanism. Beyond energy metabolism this process supports biosynthetic pathways and contributes to the maintenance of cellular function and survival. An example of glucose uptake importance includes energy provision during periods of growth or recovery highlighting its role in diverse biological contexts.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe process of glucose uptake integrates into critical biological pathways like glycolysis and the broader glucose metabolism. It starts with the activation of insulin signaling pathways which then stimulate glucose transporter translocation to the cell membrane. Proteins such as insulin receptor substrates (IRS) and PI3K\/Akt are intimately linked to these pathways facilitating signal transduction that triggers glucose uptake. These pathways not only regulate glucose levels in the body but also influence processes such as protein synthesis and cell growth.\u003cbr\u003e\nGlucose uptake malfunctions are closely associated with diabetes and metabolic syndrome. Reduced sensitivity to insulin leads to impaired GLUT4 translocation and therefore decreased glucose uptake in muscle and adipose tissues elevating blood glucose levels— a hallmark of type 2 diabetes. Moreover cancer cells exhibit altered glucose uptake where overexpression of certain GLUTs like GLUT1 allows them to satisfy increased metabolic demands thereby promoting proliferation. Aberrations in these mechanisms highlight the critical connection between glucose uptake and disease pathogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843538243753,"sku":"ab136955","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab136955","provider":"Iright","version":"1.0","type":"link"}