{"product_id":"abcam-ab137969","title":"Abcam, ab137969, Human Pancreatic Amylase ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Pancreatic Amylase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Pancreatic Amylase in Human in Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Saliva, Urine, Plasma, Milk, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026gt; 0.053 mIU\/mL,\u003cbr\u003e\nRange:0.156 - 10 mIU\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Pancreatic Amylase Human\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human pancreatic amylase in plasma, serum, urine, milk, saliva samples.\u003cbr\u003e\nA Pancreatic Amylase specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Pancreatic Amylase specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Pancreatic Amylase captured in plate.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPancreatic amylase also known as alpha-amylase or amylopsin is an enzyme with a molecular mass of approximately 55 kDa. This enzyme is expressed mainly in the pancreas and is secreted into the small intestine. Its primary mechanical function involves hydrolyzing starch into smaller carbohydrates such as maltose and dextrin. Pancreatic amylase activity is essential for the efficient digestion of dietary starch aiding in the breakdown into sugars that the body can easily absorb and utilize.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme facilitates the digestive process by catalyzing the cleavage of 14-glycosidic bonds in polysaccharides. Pancreatic amylase operates independently and not as part of a larger protein complex. Its function exclusively converts complex carbohydrates into simpler absorbable sugars. This role is essential in human metabolism as it initiates the conversion of dietary carbohydrates into forms that the intestine can further process and transport throughout the body.\u003cbr\u003e\nPathways\u003cbr\u003e\nPancreatic amylase participates in the carbohydrate digestion and absorption pathway. It interacts with other enzymes such as maltase and isomaltase which further break down the products of its enzymatic activity. This enzyme plays a core role in the pathway ensuring proper starch conversion into glucose which cells can uptake for energy. Pancreatic amylase parallels salivary amylase which begins the starch digestion process in the mouth.\u003cbr\u003e\nPancreatic amylase levels serve as a marker for pancreatitis an inflammation of the pancreas. Elevated serum levels indicate pancreatic injury or inflammation pointing to disorders that affect enzyme secretion. Chronic pancreatitis or acute pancreatitis often correlates with increased pancreatic amylase. Furthermore in certain digestive disorders like cystic fibrosis the secretion of pancreatic amylase may be disrupted indicating its pivotal role in maintaining pancreatic function and health. The enzyme's activity measurement can aid in diagnosing these conditions and monitoring their progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843631829161,"sku":"ab137969","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab137969","provider":"Iright","version":"1.0","type":"link"}