{"product_id":"abcam-ab138871","title":"Abcam, ab138871, Acetylcholinesterase Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nAcetylcholinesterase Assay Kit ab138871 uses DTNB to quantify thiocholine produced by the action of acetylcholinesterase \/ AChE activity. Readout on any colorimetric (410nm) plate reader. - Measure AChE activity in 30 minutes - Cited in \u0026gt;60 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Lysate, Plasma, Cell culture extracts, Serum, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:= 1 mU\/mL,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAcetylcholinesterase Assay Kit ab138871 provides a convenient colorimetric assay for the detection of AChE activity in blood, cell extracts and other samples from mammals and other species.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe acetylcholinesterase assay protocol uses DTNB to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. The absorption intensity of DTNB adduct (410 nm) is used to measure the amount of thiocholine formed, which is proportional to the AChE activity.\u003cbr\u003e\nThe assay will detect as little as 0.1 mU AChE in a 100 μL assay volume (1 mU\/mL).\u003cbr\u003e\nPlease note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine. However, an acetylcholinesterase inhibitor like Donepezil hydrochloride can be used as a control.\u003cbr\u003e\nAcetylcholinesterase assay protocol summary\u003cbr\u003e\n- add standards and samples to wells\u003cbr\u003e\n- add acetylthiocholine reaction mix and incubate for 10-30 min at room temp\u003cbr\u003e\n- analyze with a microplate reader\u003cbr\u003e\nPlease note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine. However, an acetylcholinesterase inhibitor like Donepezil hydrochloride can be used as a control.\u003cbr\u003e\nAlternative assay kits\u003cbr\u003e\nFor fluorometric detection, we recommend Acetylcholinesterase Assay Kit (Fluorometric-Green) (\u003cbr\u003e\nab133872\u003cbr\u003e\n) (Ex\/Em = 490\/520 nm) or Acetylcholinesterase Assay Kit (Fluorometric-Red) (\u003cbr\u003e\nab133873\u003cbr\u003e\n) (Ex\/Em = 540\/590 nm).\u003cbr\u003e\nHow other researchers are using ab138871\u003cbr\u003e\nab138871 has been used with a variety of sample types including:\u003cbr\u003e\n- Human derived mesenchymal stem cell line 1\u003cbr\u003e\n- Mouse colon tissue 2\u003cbr\u003e\n- Zebrafish Brain tissues\u003cbr\u003e\nReferences: 1 - He Y t al. 2023; 2 - Kim NY et al. 2023; 3 - Karoglu-Eravsar E at al. 2023.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAcetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAcetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nAcetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.\u003cbr\u003e\nAcetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme's interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843540504745,"sku":"ab138871","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab138871","provider":"Iright","version":"1.0","type":"link"}