{"product_id":"abcam-ab138872","title":"Abcam, ab138872, Acetylcholinesterase Assay Kit (Fluorometric - Green)","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nAcetylcholinesterase Assay KIt (Fluorometric - Green) (ab138872) is a mix-and-read assay using a Thiol Green Indicator to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. Readout on any fluorometric (Ex\/Em 490\/520 nm) plate reader. - includes Acetylcholinesterase \/ AChE enzyme positive control \/ standard\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Cell culture extracts, Whole Blood,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:= 0.1 mU\/mL,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAcetylcholinesterase Assay KIt (Fluorometric - Green) (ab138872) uses a Thiol Green Indicator to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE in blood, in cell extracts, and in other solutions.\u003cbr\u003e\nThiol Green Indicator is not fluorescent until reacted with a thiol group. It has spectral properties similar to those of fluorescein, making this assay compatible with almost every fluorescence instrument. The fluorescence intensity of Thiol Green Indicator is used to measure AChE activity. Compared to the existing thiol probes (e.g., mBBr and bBBr), Thiol Green Indicator is much more sensitive.\u003cbr\u003e\nThis product is an optimized \"mix-and-read\" assay that provides an ultrasensitive fluorometric one-step protocol to detect as little as 0.01mU AChE in a 100 μl assay volume (0.1 mU\/ml). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex\/Em = 490\/520 nm.\u003cbr\u003e\nPlease note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine.\u003cbr\u003e\nRelated AChE assay kits\u003cbr\u003e\nFor a colorimetric assay readout, use Acetylcholinesterase Assay Kit (Colorimetric) (\u003cbr\u003e\nab138871\u003cbr\u003e\nIf your plate reader has a filter to measure red fluorescence (Ex\/Em = 540\/590 nm), use Acetylcholinesterase Assay Kit (Fluorometric - Red) (\u003cbr\u003e\nab138873\u003cbr\u003e\nOther notes\u003cbr\u003e\nAcetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer's disease (AD) and myasthenia gravis.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAcetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAcetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nAcetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.\u003cbr\u003e\nAcetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme's interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855614693545,"sku":"ab138872","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab138872","provider":"Iright","version":"1.0","type":"link"}