{"product_id":"abcam-ab14734","title":"Abcam, ab14734, Anti-VDAC1\/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-VDAC1\/Porin + VDAC3 antibody [20B12AF2] (ab14734) is a mouse monoclonal antibody detecting VDAC1\/Porin in  Western Blot, Flow Cytometry, ICC\/IF . Suitable for  Cow, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 580 publications - Trusted since 2005\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:20B12AF2,\u003cbr\u003e\nIsotype:IgG2b,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Cow, Human,\u003cbr\u003e\nApplications:WB, Flow Cyt, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThis antibody clone [20B12AF2] is manufactured by Abcam.\u003cbr\u003e\nIf you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact\u003cbr\u003e\norders@abcam.com\u003cbr\u003e\nor you can find further information\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-VDAC1\/Porin + VDAC3 antibody [20B12AF2] (ab14734) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC\/IF, WB in cow, human, mouse, rat samples.\u003cbr\u003e\nAnti-VDAC1\/Porin + VDAC3 antibody [20B12AF2] (ab14734) specifically detects VDAC1\/Porin + VDAC3 (UniProt ID: P21796; Molecular weight: 31kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-VDAC1\/Porin + VDAC3 antibody [20B12AF2] (ab14734) has high sensitivity and specificity.\u003cbr\u003e\nThe specificity of Anti-VDAC1\/Porin + VDAC3 antibody [20B12AF2] (ab14734) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-VDAC1\/Porin + VDAC3 antibody [20B12AF2] (ab14734) has been cited over 584 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-VDAC1\/Porin + VDAC3 antibody [20B12AF2] (ab14734) has 51 independent reviews from customers.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-IgG fraction, Purification notes-Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 0.88% Sodium chloride, 0.36% HEPES, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nVDAC1 also known as Porin or Porin Hub and VDAC3 are part of the voltage-dependent anion-selective channel family located in the outer mitochondrial membrane. These proteins form channels that regulate the flow of metabolites and ions. With each weighing approximately 31 kDa they are pivotal in cellular energy homeostasis. Expression of VDAC1 and VDAC3 is observed in various tissues with significant presence in energy-demanding organs such as the brain and muscles.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThese voltage-dependent channels mediate the exchange of ATP and ions facilitating mitochondrial function. VDAC1\/Porin often interacts with other proteins to form complexes that influence cell viability by regulating apoptosis. VDAC3 while similar in structure demonstrates distinct regulatory functions and is expressed alongside VDAC1 in specific contexts contributing to overall mitochondrial efficiency.\u003cbr\u003e\nPathways\u003cbr\u003e\nThese channels are deeply embedded within the cellular bioenergetics and apoptotic pathways. They interact closely with anti-apoptotic proteins like Bcl-2 influencing cell survival. Additionally VDAC1 and VDAC3 participate in oxidative phosphorylation ensuring efficient energy production by controlling the metabolites' access to the mitochondria.\u003cbr\u003e\nDysfunction of VDAC1 and VDAC3 relates strongly to neurodegenerative diseases such as Alzheimer’s. Misregulation of VDAC1 is associated with altered apoptotic processes linked to cancer. VDAC1\/Porin connects with proteins like Bax and caspases in these conditions highlighting their role in the pathogenesis through modulated apoptotic signaling.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844026028201,"sku":"ab14734","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab14734","provider":"Iright","version":"1.0","type":"link"}