{"product_id":"abcam-ab150675","title":"Abcam, ab150675, Luxol Fast Blue Stain Kit (Myelin Stain)","description":"\u003cp\u003eSize: 30mL \/ 100Test \/ 125mL\u003cbr\u003e\nLuxol fast blue stain kit ab150675 is used for staining myelin\/myelinated axons and Nissl bodies in formalin-fixed, paraffin-embedded tissue, as well as frozen tissue. - Used to identify basic neuronal structure in brain or spinal cord sections - Simple and fast protocol with ready-to-use solutions - Cited in over 20 publications\u003cbr\u003e\nProduct details:\u003cbr\u003e\nLuxol Fast Blue Stain Kit (Myelin Stain) ab150675 is designed for staining myelin\/myelinated axons and Nissl bodies on formalin fixed, paraffin-embedded tissue as well as frozen tissue. This product is used for identifying the basic neuronal structure in brain or spinal cord sections.\u003cbr\u003e\nLuxol Fast Blue staining protocol summary:\u003cbr\u003e\n- deparaffinze sections if necessary and hydrate in water\u003cbr\u003e\n- incubate in Luxol Fast Blue solution for 24 hr at room temp, or 2 hr at 60°C\u003cbr\u003e\n- rinse in water\u003cbr\u003e\n- differentiate by dipping in lithium carbonate solution\u003cbr\u003e\n- differentiate further by dipping in alcohol reagent\u003cbr\u003e\n- rinse in water\u003cbr\u003e\n- incubate in Cresyl Echt Violet for 2-5 min\u003cbr\u003e\n- rinse in water\u003cbr\u003e\n- dehydrate in absolute alcohol\u003cbr\u003e\n- clear and mount\u003cbr\u003e\nOther products for staining tissue sections\u003cbr\u003e\nFind more kits and reagents in the\u003cbr\u003e\nspecial stains guide\u003cbr\u003e\n, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the\u003cbr\u003e\nIHC kits and reagents guide\u003cbr\u003e\nStaining Interpretation\u003cbr\u003e\nMyelinated Fibers\u003cbr\u003e\nNissil Substance\u003cbr\u003e\nViolet\u003cbr\u003e\nNerve Cells\u003cbr\u003e\nViolet\u003cbr\u003e\nControl Tissue:\u003cbr\u003e\nCerebral Cortex, Spinal Cord.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nPlease note, the 125 mL selling size, was previously described as 100 Test. There has been no change to the physical product.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMyelin is a lipid-rich substance that insulates nerve fibers in both the central nervous system (CNS) and peripheral nervous system (PNS). It is mainly composed of protein and lipids contributing to its unique insulative properties. Proteolipid protein (PLP) in the CNS and myelin protein zero (MPZ) in the PNS serve as major structural components. Oligodendrocytes in the CNS and Schwann cells in the PNS specifically express this sheath around axons. Myelin does not have a specific mass as it describes the structure rather than a single protein or molecule. Its role in speeding up electrical signals along neurons is recognized by various staining techniques such as Luxol Fast Blue and myelin staining which are helpful in visualizing its structure.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMyelin plays an important role in the nervous system by facilitating rapid signal transmission between neurons. Myelin enables saltatory conduction where impulses jump from one Node of Ranvier to the next significantly increasing signal speed and efficiency. Its contribution to the formation of the myelin sheath involves interactions with other proteins forming a complex and stable structure. For instance proteins like myelin basic protein (MBP) and PLP integrate into the myelin membrane to ensure compact and stable layering.\u003cbr\u003e\nPathways\u003cbr\u003e\nMyelination integrates closely with the development and function of nerve axons. The myelination process involves the Akt\/mTOR signaling pathway which promotes myelin growth and sustains myelin sheath maintenance. Other pathways such as the Nrg1\/ErbB pathway regulate Schwann cell function and influence axon myelination. Key proteins related through these pathways include ErbB receptors which interact with neuregulin-1 to influence myelin formation and maintenance.\u003cbr\u003e\nMyelin degradation significantly impacts multiple sclerosis (MS) and Charcot-Marie-Tooth disease (CMT). In MS demyelination results from an autoimmune response against myelin leading to neurological dysfunctions. Disorders like CMT involve mutations in genes coding for myelin proteins such as PMP22 causing peripheral nerve damage. Both of these conditions highlight the essential connection between myelin and proper nervous system operation with potential disorders disrupting signaling and leading to a range of neurological symptoms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843555774633,"sku":"ab150675","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab150675","provider":"Iright","version":"1.0","type":"link"}