{"product_id":"abcam-ab154799","title":"Abcam, ab154799, Anti-LEPRE1\/P3H1 antibody [EPR10193(B)]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal LEPRE1\/P3H1 antibody. Suitable for WB, I-ELISA, ICC\/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide - Human samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR10193(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, Flow Cyt (Intra), WB, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLEPRE1 also known as P3H1 or prolyl 3-hydroxylase 1 is an enzyme involved in collagen synthesis with a molecular mass of approximately 71 kDa. It is a member of the prolyl hydroxylase family and carries out 3-hydroxylation of proline residues in collagen. The enzyme localizes largely to the endoplasmic reticulum where it contributes to the post-translational modification of collagen. LEPRE1 is ubiquitously expressed with higher concentrations observed in tissues rich in collagen such as skin bone and cartilage.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLEPRE1 plays a fundamental role in the maturation of collagen which is an important structural protein in connective tissues. This enzyme functions as a part of the collagen prolyl 3-hydroxylation complex which includes cartilage-associated protein (CRTAP) and cyclophilin B (PPIB). The complex is responsible for the correct folding and stability of collagen molecules ensuring the proper formation of the extracellular matrix. This structural role is essential for maintaining tissue integrity and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nLEPRE1 is important in the collagen biosynthesis and modifying pathways. It interacts with other biosynthesis enzymes like lysyl oxidase and procollagen-proline dioxygenase. These proteins coordinate to execute post-translational modifications which stabilize the collagen triple helix structure. Pirh2 and Hsp47 act along with LEPRE1 in similar pathways helping in folding and secretion of collagen.\u003cbr\u003e\nLEPRE1 mutations have been linked to connective tissue disorders most notably osteogenesis imperfecta a genetic disorder causing brittle bones. Such mutations impair collagen modification leading to defective collagen formation and bone fragility. Additionally dysregulation of LEPRE1 activity appears connected to disorder Ehlers-Danlos syndrome noted for joint hypermobility and skin elasticity issues. COL1A1 and COL1A2 two primary components of type I collagen often associate with LEPRE1 in disease contexts as mutations in these collagens lead to similar clinical manifestations.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844187574441,"sku":"ab154799","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab154799","provider":"Iright","version":"1.0","type":"link"}