{"product_id":"abcam-ab155437","title":"Abcam, ab155437, Human Notch-1 ELISA Kit (TAN-1)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Notch-1 ELISA Kit (TAN-1) is a Sandwich (quantitative) ELISA for the measurement of Human Notch-1 (TAN-1) in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 20 pg\/mL,\u003cbr\u003e\nRange:0.029 - 7 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Notch-1 (TAN-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay designed for the quantitative measurement of Human Notch-1 (TAN-1) in serum, plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human Notch-1(TAN-1) coated on a 96-well plate. Standards and samples are pipetted into the wells and Notch-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated Anti-Human Notch-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Notch-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nOptimization may be required with urine samples.\u003cbr\u003e\nThis kit can detect extracellular domain of NOTCH1.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNotch-1 also known as TAN-1 is a transmembrane receptor protein with a molecular mass of approximately 300 kDa. It is part of the Notch family and is extensively expressed in various tissues including the brain heart and immune cells. The receptor plays an important role in cell-fate determination by mediating communication between adjacent cells through its interaction with notch ligands like Delta and Jagged.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNotch-1 directly influences cell differentiation proliferation and apoptosis by participating in the Notch signaling pathway. It is part of a complex with Notch ligands and the γ-secretase enzyme which facilitates its cleavage and activation. This signaling assists in controlling the balance between cell types in the developing organism and adult tissue homeostasis making it important for proper function and organism development.\u003cbr\u003e\nPathways\u003cbr\u003e\nNotch-1 is involved in regulating the Notch signaling pathway which impacts gene expression once the intracellular domain translocates to the nucleus. Additionally it interacts with proteins such as Mastermind-like proteins to activate transcription of target genes. Notch-1 also intersects with the Wnt signaling pathway suggesting crosstalk between these important pathways in maintaining cell fate decisions and tissue development.\u003cbr\u003e\nNotch-1 has connections to T-cell acute lymphoblastic leukemia as mutations in Notch-1 often lead to uncontrolled cell proliferation. The receptor is also linked to breast cancer where abnormal Notch-1 signaling encourages tumor progression. In these disorders Notch-1 interacts with proteins such as NF-kB which influences cancer cell survival and proliferation making Notch-1 a significant target for therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855635206313,"sku":"ab155437","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab155437","provider":"Iright","version":"1.0","type":"link"}