{"product_id":"abcam-ab155891","title":"Abcam, ab155891, Total Carbohydrate Assay Kit - Quantification","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nTotal Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Suspension cells, Tissue Extracts, Adherent cells, Food samples, Drinks,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTotal Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe carbohydrate assay protocol is based on the phenol - sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural. These react with the developer to form a chromogen that can be quantified by measuring the absorbance at 490 nm.\u003cbr\u003e\nTotal carbohydrate assay protocol summary:\u003cbr\u003e\n- Add samples and glucose standards to wells\u003cbr\u003e\n- Add concentrated sulfuric acid and incubate for 15 min at 90°C\u003cbr\u003e\n- Add developer and incubate for 5 min\u003cbr\u003e\nHow other researchers are using ab155891\u003cbr\u003e\nTotal Carbohydrate Assay Kit\u003cbr\u003e\nab155901\u003cbr\u003e\nhas been used in a variety of sample type including:\u003cbr\u003e\nSHK-1 cell line\u003cbr\u003e\nBlood samples from mice with metabolic syndrome\u003cbr\u003e\nLattuce seeds extracts\u003cbr\u003e\nReferences: 1-Martinez D et al. 2022, 2-Greco C et al. 2020, 3-Rawashdeh Y et al. 2020\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee an alternative kit to quantify carbohydrates:\u003cbr\u003e\nHigh Sensitivity Carbohydrate Assay Kit (Colorimetric)\u003cbr\u003e\nab284543\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K645 Biovision Total Carbohydrate Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nCarbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843546402985,"sku":"ab155891","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab155891","provider":"Iright","version":"1.0","type":"link"}