{"product_id":"abcam-ab156066","title":"Abcam, ab156066, SIRT2 Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nSIRT2 Activity Assay Kit (Fluorometric) (ab156066) detects deacetylase activity of recombinant SIRT2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's SIRT2 Activity Assay Kit (Fluorometric) (ab156066) detects deacetylase activity of recombinant SIRT2.\u003cbr\u003e\nPrimarily, Abcam's SIRT2 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT2 inhibitors or activators using recombinant SIRT2 or purified SIRT2.\u003cbr\u003e\nApplications for this kit include:\u003cbr\u003e\n1. Screening inhibitors or activators of SIRT2.\u003cbr\u003e\n2. Detecting the effects of pharmacological agents on SIRT2.\u003cbr\u003e\nHistone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.\u003cbr\u003e\nHDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.\u003cbr\u003e\nClasses I, IIA and IIB are considered \"classical\" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSIRT2 also known as Sirtuin 2 is a member of the sirtuin family of proteins which are NAD+-dependent deacetylases known for regulating various cellular processes. SIRT2 has a molecular mass of approximately 43 kDa. Its expression is prominent in the cytoplasm but can also be found in the nucleus of cells particularly in the brain and liver tissues. The protein deacetylates various substrates influencing cellular processes including aging metabolism and neurodegeneration.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSIRT2 contributes to the regulation of cellular homeostasis and stress responses. This protein acts as a deacetylase modulating the acetylation status of key proteins and participates in various signaling pathways. It functions as part of a larger protein complex and interacts with substrates that include tubulin and histones. These interactions help SIRT2 control processes such as cell cycle differentiation and proliferation indicating its importance in maintaining normal cellular function.\u003cbr\u003e\nPathways\u003cbr\u003e\nSIRT2 plays an essential role in the insulin signaling pathway and the FoxO transcription factor pathway. In these pathways SIRT2 interacts with other sirtuins like SIRT1 which helps regulate metabolic homeostasis and oxidative stress responses. SIRT2's activity in deacetylating proteins such as p53 impacts cellular responses to DNA damage and genomic stability further integrating it into critical regulatory networks within the cell.\u003cbr\u003e\nSIRT2 relates to neurodegenerative diseases like Parkinson's disease and metabolic disorders such as obesity. Its involvement in deacetylating alpha-synuclein links SIRT2 to Parkinson's disease pathogenesis. In metabolic disorders abnormalities in SIRT2 activity can affect glucose and lipid metabolism connecting it with proteins like acetyl-CoA carboxylase. Understanding SIRT2's function offers potential therapeutic targets for these conditions and highlights its importance in disease regulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843630649513,"sku":"ab156066","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab156066","provider":"Iright","version":"1.0","type":"link"}