{"product_id":"abcam-ab156910","title":"Abcam, ab156910, KDM6A\/ KDM6B Activity Quantification Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 48Test \/ 96Test\u003cbr\u003e\nKDM6A\/KDM6B Activity Quantification Assay Kit (Colorimetric) (ab156910) is a complete set of optimized reagents, designed for measuring activity\/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Nuclear Extracts, Purified protein, Cell culture extracts, Tissue Extracts, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:\u0026gt; 10000 ng,\u003cbr\u003e\nRange:1 - 20000 ng,\u003cbr\u003e\nAssay time:3h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKDM6A\/KDM6B Activity Quantification Assay Kit (Colorimetric) (ab156910) is a complete set of optimized reagents, designed for measuring activity\/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. There are currently a limited number of methods used for detecting KDM6A\/KDM6B activity\/inhibition.\u003cbr\u003e\nThe traditional method is based on the measurement of formaldehyde release, a by-product of KDM6A\/KDM6B enzymatic reaction, and has significant weaknesses: (1) large amounts (at μg level) of substrate and enzyme are required; (2) nuclear extracts from cell\/tissues cannot be used; (3) redox-sensitive KDM6A\/KDM6B inhibitiors are not suitable for testing with such methods; (4) high intereference by SDS, DMSO, thiol-containing chemicals, and ions, which are often contained in enzyme solutions, tested compound solvents, and assay buffers; and (5) less accurate than a direct measurement of KDM6A\/KDM6B-converted demethylated products.\u003cbr\u003e\nKDM6A\/KDM6B Activity Quantification Assay Kit (Colorimetric) (ab156910) addresses these issues.\u003cbr\u003e\nLysine specific demethylase 6A (KDM6A\/UTX) and lysine specific demethylase 6B (KDM6B\/JMJD3) demethylase lysine 27 on histone H3, thereby playing a central role in histone code. KDM6A and KDM6B are JmjC (Jumonji)-domain-containing proteins and catalyze the removal of di- and tri-methylation from histone H3-K27 by using a hydroxylation reaction with iron and alpha-glutarate as cofactors.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843651489961,"sku":"ab156910","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab156910","provider":"Iright","version":"1.0","type":"link"}