{"product_id":"abcam-ab157489","title":"Abcam, ab157489, Anti-LRRC8A antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRabbit Polyclonal LRRC8A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LRRC8A aa 600 to C-terminus.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human LRRC8A aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8IWT6\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Lyophilized, Reconstitution-Reconstitute in 200 µL of water, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe LRRC8A protein also known as SWELL1 plays a mechanical role as a component of the volume-regulated anion channel (VRAC). It has a molecular mass of approximately 104 kDa. The protein functions in transporting chloride ions across the plasma membrane helping to maintain cellular osmotic balance. LRRC8A is widely expressed in many tissues with high levels in the kidney heart and brain indicating its broad functional relevance across different organ systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLRRC8A forms heteromeric complexes with related proteins LRRC8B LRRC8C LRRC8D and LRRC8E. These complexes constitute the VRAC channels which are important for regulating cell volume in response to osmotic changes a process called regulatory volume decrease. LRRC8A's involvement in this process is essential for cell survival under fluctuating environmental conditions as it prevents cellular swelling and lysis by allowing ions and osmolytes to exit the cell.\u003cbr\u003e\nPathways\u003cbr\u003e\nResearchers have linked LRRC8A to the PI3K-AKT signaling pathway and the apoptotic pathway. In the context of the PI3K-AKT pathway LRRC8A contributes to cell survival and growth regulation interacting with proteins like AKT. Within the apoptotic pathway through VRAC activity LRRC8A plays a role in apoptosis regulation facilitating the release of apoptogenic factors that trigger programmed cell death when necessary.\u003cbr\u003e\nResearchers have connected LRRC8A to conditions such as ischemia-reperfusion injury and cancer. In ischemia-reperfusion injury inadequate regulation by LRRC8A can lead to excessive cell swelling and damage upon reperfusion making it a potential therapeutic target. In cancer its role in cell growth pathways implicates LRRC8A in the uncontrolled proliferation seen in malignancies. Its association with VRAC partners like LRRC8D underpins its involvement in these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855814414505,"sku":"ab157489","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab157489","provider":"Iright","version":"1.0","type":"link"}