{"product_id":"abcam-ab157716","title":"Abcam, ab157716, Mouse Hemopexin ELISA Kit (HPX)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse Hemopexin ELISA Kit (HPX) is a sandwich ELISA designed to quantify Mouse Hemopexin (HPX) with a sensitivity of 4.5092 ng\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 12.5 - 400 ng\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Serum, Other biological fluids,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 4.5092 ng\/mL,\u003cbr\u003e\nRange:12.5 - 400 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Hemopexin ELISA Kit (HPX) ab157716 is a sandwich ELISA to measure Mouse Hemopexin (HPX) in serum, plasma, other biological fluids with a sensitivity of 4.5092 ng\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- Hemopexin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemopexin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemopexin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemopexin in the test sample. The quantity of Hemopexin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nMouse Hemopexin ELISA Kit (HPX) ab157716 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature.\u003cbr\u003e\n2. Aspirate and wash each well.\u003cbr\u003e\n3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature\u003cbr\u003e\n4. Aspirate and wash each well.\u003cbr\u003e\n5. Add Chromogen Substrate Solution to each well.\u003cbr\u003e\n6. Immediately begin recording the color development\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHemopexin also known as HPX is a glycoprotein that transports heme in the blood. It has a molecular weight of approximately 57 kDa and is primarily expressed in the liver. Hemopexin binds to free heme with high affinity which prevents oxidative damage and conserves iron. It transports the heme to hepatocytes for breakdown and recycling protecting cells from iron-mediated toxicity and oxidative stress.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe hemopexin function relates to protecting tissues from the harmful effects of free heme. Hemopexin does not work as part of a complex in this process but mainly acts independently. Its high binding affinity plays an important role in maintaining heme homeostasis in the plasma and tissues. By controlling heme levels hemopexin supports overall cellular health and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe hemopexin molecular weight allows it to fit effectively within the heme metabolism and iron recycling pathways. This protein plays a significant role by delivering heme to the liver and facilitating its degradation by heme oxygenase. Hemopexin interacts closely with heme oxygenase and albumin two proteins important for the management of heme and iron levels in the body.\u003cbr\u003e\nSeveral hemopexin functions contribute to the understanding of conditions such as hemolytic anemia and acute liver injury. In hemolytic anemia hemopexin helps mitigate the effects of increased heme levels due to excessive red blood cell breakdown. In the context of liver injury the protein assists in the detoxification process and supports hepatic function. Hemopexin-related proteins such as heme oxygenase link these diseases to hemopexin by their roles in managing oxidative stress and heme catabolism.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843558625449,"sku":"ab157716","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab157716","provider":"Iright","version":"1.0","type":"link"}