{"product_id":"abcam-ab157729","title":"Abcam, ab157729, Rat Alpha 1 Acid Glycoprotein \/ AGP ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nRat Alpha 1 Acid Glycoprotein \/ AGP ELISA Kit is a sandwich ELISA designed to quantify Rat Alpha 1 Acid Glycoprotein \/ AGP with a sensitivity of 2.1663 ng\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 12.5 - 400 ng\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Serum,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 2.1663 ng\/mL,\u003cbr\u003e\nRange:12.5 - 400 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nRat Alpha 1 Acid Glycoprotein \/ AGP ELISA Kit ab157729 is a sandwich ELISA to measure Rat Alpha 1 Acid Glycoprotein \/ AGP in serum, plasma with a sensitivity of 2.1663 ng\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn this assay the Alpha 1 Acid Glycoprotein \/ AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein \/ AGP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein \/ AGP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein \/ AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein \/ AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein \/ AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein \/ AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nRat Alpha 1 Acid Glycoprotein \/ AGP ELISA Kit ab157729 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature.\u003cbr\u003e\n2. Aspirate and wash each well.\u003cbr\u003e\n3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature\u003cbr\u003e\n4. Aspirate and wash each well.\u003cbr\u003e\n5. Add Chromogen Substrate Solution to each well.\u003cbr\u003e\n6. Immediately begin recording the color development\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body's inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.\u003cbr\u003e\nAGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843644674217,"sku":"ab157729","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab157729","provider":"Iright","version":"1.0","type":"link"}