{"product_id":"abcam-ab167154","title":"Abcam, ab167154, Anti-TRIM25\/EFP antibody [EPR7315]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-TRIM25\/EFP antibody [EPR7315] (ab167154) is a rabbit monoclonal antibody detecting TRIM25\/EFP in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7315,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, Flow Cyt (Intra), IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-TRIM25\/EFP antibody [EPR7315] (ab167154) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of TRIM25\/EFP?\u003cbr\u003e\nAnti-TRIM25\/EFP [EPR7315] (ab167154) specifically detects a band for TRIM25\/EFP (UniProt: Q14258) at a molecular weight of 71kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-TRIM25\/EFP [EPR7315] (ab167154) was first used in a scientific publication in 2013 and has been cited over 30 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-TRIM25\/EFP antibody [EPR7315] (ab167154) has been confirmed by Western blot testing in TRIM25 Knockout HAP1 cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR7315] also available for your convenience: ab167154, Alexa Fluor® 488 -\u003cbr\u003e\nab200781\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab200788\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab232356\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTRIM25 also known as EFP is a protein involved in antiviral response mechanisms. TRIM25 has a molecular mass of approximately 70 kDa and is part of the TRIM (Tripartite Motif) family which contains three distinct zinc-binding domains followed by a coiled-coil region. This protein exhibits strong expression in a variety of tissues including lung liver and intestines. As a member of the E3 ubiquitin ligase enzyme family TRIM25 plays a direct role in protein ubiquitination which tags specific proteins for degradation by the proteasome.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTRIM25 functions as a significant modulator of the innate immune response against viral infections. In cells it acts within the RIG-I signaling complex directly promoting the RIG-I-MAVS pathway that detects viral RNA. TRIM25 mediates the ubiquitination of RIG-I allowing for its proper activation and subsequent signal transduction facilitating a robust antiviral state. TRIM25's role extends beyond antiviral defenses to include regulatory functions in cell proliferation and differentiation.\u003cbr\u003e\nPathways\u003cbr\u003e\nTRIM25 engages actively in the innate immune signaling and JAK-STAT pathways. It interacts and cooperates with RIG-I in the recognition and response to viral tRNA leading to the production of interferons that establish an antiviral environment. Furthermore TRIM25 also connects with proteins like TRIM21 which further engages the immune response. This interaction enhances the overall ability of cells to fend off viral pathogens efficiently.\u003cbr\u003e\nAltered TRIM25 function is associated with conditions like systemic lupus erythematosus (SLE) and various cancers. In the context of SLE TRIM25's regulatory role in innate immunity can affect the severity and progression of this autoimmune disorder. Additionally in some cancers abnormal TRIM25 expression can influence cell cycle regulation and metastasis. Through its ubiquitination activities TRIM25 interacts directly with proteins such as p53 affecting tumor suppression pathways and contributing to the complexity of disease development and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844040118441,"sku":"ab167154","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab167154","provider":"Iright","version":"1.0","type":"link"}